FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076131544> ?p ?o ?g. }

• W2076131544 endingPage "1164" @default.
• W2076131544 startingPage "1159" @default.
• W2076131544 abstract "To investigate the role of transcription factor c-Ets1 in cyclin D3 expression and its effects on the proliferation of umbilical cord hematopoietic cells.Cyclin D3 promoter deletion constructs were generated and transfected into CD34(+) cells. Dual luciferase reporter assays and TFSEARCH software were used to identify negative regulatory domains and to predict putative transcription factors involved in cyclin D3 downregulation. Expression of c-Ets1 in CD34(+) cells was detected using electrophoretic mobility shift and super shift assays. Point mutants of c-Ets1 binding sites were constructed. The wild-type c-Ets1 and the mutant promoter constructs were co-transfected into CD34(+) cells to determine the promoter activity. The impact of c-Ets1 expression on the proliferation of CD34(+) cells was assessed using MTT assay.Nine cyclin D3 promoter deletion constructs were generated. A negative regulatory domain containing c-Ets1 binding sites was identified between -439 bp and -362 bp. Transfection of the promoter deletion constructs containing mutant c-Ets1 binding sites enhanced cyclin D3 promoter activity. However, the opposite results were observed when CD34(+) cells were co-transfected with wildtype c-Ets1 and its promoter deletion constructs. The overexpression of c-Ets1 could suppress cyclin D3 mRNA and protein levels. In addition, it inhibits the proliferation of CD34(+) cells.c-Ets1 functions as a negative transcription factor, down-regulating the expression of cyclin D3, which leads to inhibition of CD34(+) cell proliferation." @default.
• W2076131544 created "2016-06-24" @default.
• W2076131544 creator A5025704945 @default.
• W2076131544 creator A5032287435 @default.
• W2076131544 creator A5041861004 @default.
• W2076131544 creator A5051764626 @default.
• W2076131544 creator A5075170039 @default.
• W2076131544 creator A5087880097 @default.
• W2076131544 date "2011-08-15" @default.
• W2076131544 modified "2023-10-06" @default.
• W2076131544 title "Negative regulation of cyclin D3 expression by trans-cription factor c-Ets1 in umbilical cord hematopoietic cells" @default.
• W2076131544 cites W1883290480 @default.
• W2076131544 cites W1971497482 @default.
• W2076131544 cites W1999656457 @default.
• W2076131544 cites W2007137412 @default.
• W2076131544 cites W2016907191 @default.
• W2076131544 cites W2018992583 @default.
• W2076131544 cites W2020330574 @default.
• W2076131544 cites W2033172704 @default.
• W2076131544 cites W2039040881 @default.
• W2076131544 cites W2047277395 @default.
• W2076131544 cites W2048004395 @default.
• W2076131544 cites W2058127668 @default.
• W2076131544 cites W2064772366 @default.
• W2076131544 cites W2072885506 @default.
• W2076131544 cites W2081646565 @default.
• W2076131544 cites W2091332908 @default.
• W2076131544 cites W2135174193 @default.
• W2076131544 cites W2253277805 @default.
• W2076131544 doi "https://doi.org/10.1038/aps.2011.41" @default.
• W2076131544 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4003296" @default.
• W2076131544 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21841808" @default.
• W2076131544 hasPublicationYear "2011" @default.
• W2076131544 type Work @default.
• W2076131544 sameAs 2076131544 @default.
• W2076131544 citedByCount "8" @default.
• W2076131544 countsByYear W20761315442012 @default.
• W2076131544 countsByYear W20761315442015 @default.
• W2076131544 countsByYear W20761315442017 @default.
• W2076131544 countsByYear W20761315442018 @default.
• W2076131544 countsByYear W20761315442021 @default.
• W2076131544 crossrefType "journal-article" @default.
• W2076131544 hasAuthorship W2076131544A5025704945 @default.
• W2076131544 hasAuthorship W2076131544A5032287435 @default.
• W2076131544 hasAuthorship W2076131544A5041861004 @default.
• W2076131544 hasAuthorship W2076131544A5051764626 @default.
• W2076131544 hasAuthorship W2076131544A5075170039 @default.
• W2076131544 hasAuthorship W2076131544A5087880097 @default.
• W2076131544 hasBestOaLocation W20761315441 @default.
• W2076131544 hasConcept C104317684 @default.
• W2076131544 hasConcept C109159458 @default.
• W2076131544 hasConcept C11882975 @default.
• W2076131544 hasConcept C120089663 @default.
• W2076131544 hasConcept C153911025 @default.
• W2076131544 hasConcept C16438837 @default.
• W2076131544 hasConcept C183978625 @default.
• W2076131544 hasConcept C2780538058 @default.
• W2076131544 hasConcept C2781403221 @default.
• W2076131544 hasConcept C28328180 @default.
• W2076131544 hasConcept C29537977 @default.
• W2076131544 hasConcept C54009773 @default.
• W2076131544 hasConcept C54355233 @default.
• W2076131544 hasConcept C81885089 @default.
• W2076131544 hasConcept C86339819 @default.
• W2076131544 hasConcept C86803240 @default.
• W2076131544 hasConcept C95444343 @default.
• W2076131544 hasConceptScore W2076131544C104317684 @default.
• W2076131544 hasConceptScore W2076131544C109159458 @default.
• W2076131544 hasConceptScore W2076131544C11882975 @default.
• W2076131544 hasConceptScore W2076131544C120089663 @default.
• W2076131544 hasConceptScore W2076131544C153911025 @default.
• W2076131544 hasConceptScore W2076131544C16438837 @default.
• W2076131544 hasConceptScore W2076131544C183978625 @default.
• W2076131544 hasConceptScore W2076131544C2780538058 @default.
• W2076131544 hasConceptScore W2076131544C2781403221 @default.
• W2076131544 hasConceptScore W2076131544C28328180 @default.
• W2076131544 hasConceptScore W2076131544C29537977 @default.
• W2076131544 hasConceptScore W2076131544C54009773 @default.
• W2076131544 hasConceptScore W2076131544C54355233 @default.
• W2076131544 hasConceptScore W2076131544C81885089 @default.
• W2076131544 hasConceptScore W2076131544C86339819 @default.
• W2076131544 hasConceptScore W2076131544C86803240 @default.
• W2076131544 hasConceptScore W2076131544C95444343 @default.
• W2076131544 hasIssue "9" @default.
• W2076131544 hasLocation W20761315441 @default.
• W2076131544 hasLocation W20761315442 @default.
• W2076131544 hasLocation W20761315443 @default.
• W2076131544 hasLocation W20761315444 @default.
• W2076131544 hasOpenAccess W2076131544 @default.
• W2076131544 hasPrimaryLocation W20761315441 @default.
• W2076131544 hasRelatedWork W1507530325 @default.
• W2076131544 hasRelatedWork W1533846472 @default.
• W2076131544 hasRelatedWork W1982298196 @default.
• W2076131544 hasRelatedWork W1990427876 @default.
• W2076131544 hasRelatedWork W2039496237 @default.
• W2076131544 hasRelatedWork W2055406246 @default.
• W2076131544 hasRelatedWork W2073234210 @default.
• W2076131544 hasRelatedWork W2109557991 @default.

• next