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• W2076132533 startingPage "247" @default.
• W2076132533 abstract "Powdery mildew is an important disease of wheat caused by the obligate biotrophic fungus Blumeria graminis f. sp. tritici. This pathogen invades exclusively epidermal cells after penetrating directly through the cell wall. Because powdery mildew colonizes exclusively epidermal cells, it is of importance not only to identify genes which are activated, but also to monitor tissue specificity of gene activation. Acquired resistance of wheat to powdery mildew can be induced by a previous inoculation with the non-host pathogen B. graminis f. sp. hordei, the causal agent of barley powdery mildew. The establishment of the resistant state is accompanied by the activation of genes. Here we report the tissue-specific cDNA-AFLP analysis and cloning of transcripts accumulating 6 and 24 h after the resistance-inducing inoculation with B. graminis f. sp. hordei. A total of 25,000 fragments estimated to represent about 17,000 transcripts were displayed. Out of these, 141 transcripts, were found to accumulate after Bgh inoculation using microarray hybridization analysis. Forty-four accumulated predominantly in the epidermis whereas 76 transcripts accumulated mostly in mesophyll tissue." @default.
• W2076132533 created "2016-06-24" @default.
• W2076132533 creator A5011160102 @default.
• W2076132533 creator A5019629618 @default.
• W2076132533 creator A5084203689 @default.
• W2076132533 creator A5086591994 @default.
• W2076132533 date "2005-05-01" @default.
• W2076132533 modified "2023-10-16" @default.
• W2076132533 title "Analysis of epidermis- and mesophyll-specific transcript accumulation in powdery mildew-inoculated wheat leaves" @default.
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• W2076132533 doi "https://doi.org/10.1007/s11103-005-3099-9" @default.
• W2076132533 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16027977" @default.
• W2076132533 hasPublicationYear "2005" @default.
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• W2076132533 citedByCount "36" @default.

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