FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076142444> ?p ?o ?g. }

• W2076142444 abstract "Despite advances in understanding the cell biology of glycoinositol phospholipid (GPI)-anchored proteins in cultured cells, the in vivo functions of GPI anchors have remained elusive. We have focused on Drosophila acetylcholinesterase (AChE) as a model GPI-anchored protein that can be manipulated in vivo with sophisticated genetic techniques. In Drosophila, AChE is found only as a GPI-anchored G2 form encoded by the Ace locus on the third chromosome. To pursue our goal of replacing wild-type GPI-anchored AChE with forms that have alternative anchor structures in transgenic files, we report the construction of two secreted forms of Drosophila AChE (SEC1 and SEC2) and a chimeric form (TM-AChE) anchored by the transmembrane and cytoplasmic domains of herpes simplex virus type 1 glycoprotein C. To confirm that the biochemical properties of these AChEs were unchanged from GPI-AChE except as predicted, we made stably transfected Drosophila Schneider Line 2(S2) cells expressing each of the four forms. TM-AChE, SEC1, and SEC2 had the same catalytic activity and quaternary structure as wild type. TM-AChE was expressed as an amphiphilic membrane-bound protein resistant to an enzyme that cleaves GPI-AChE (phosphatidylinositol-specific phospholipase C), and the same percentage of TM-AChE and GPI-AChE was on the cell surface according to immunofluorescence and pharmacological data. SEC1 and SEC2 were constructed by truncating the C-terminal signal peptide initially present in GPI-AChE: in SEC1 the last 25 residues of this 34-residue peptide were deleted while in SEC2 the last 29 were deleted. Both SEC1 and SEC2 were efficiently secreted and are very stable in culture medium; with one cloned SEC1-expressing line, AChE accumulated to as high as 100 mg/liter. Surprisingly, 5-10% of SEC1 was attached to a GPI anchor, but SEC2 showed no GPI anchoring. Since no differences in catalytic activity were observed among the four AChEs, and since the same percentage of GPI-AChE and TM-AChE were on the cell surface, we contend that in vivo experiments in which GPI-AChE is replaced can be interpreted solely on the basis of the altered anchoring domain." @default.
• W2076142444 created "2016-06-24" @default.
• W2076142444 creator A5002695228 @default.
• W2076142444 creator A5067631894 @default.
• W2076142444 date "1996-04-01" @default.
• W2076142444 modified "2023-09-23" @default.
• W2076142444 title "Construction and characterization of secreted and chimeric transmembrane forms of Drosophila acetylcholinesterase: a large truncation of the C-terminal signal peptide does not eliminate glycoinositol phospholipid anchoring." @default.
• W2076142444 cites W1498646052 @default.
• W2076142444 cites W1515505902 @default.
• W2076142444 cites W1529387078 @default.
• W2076142444 cites W1531300619 @default.
• W2076142444 cites W1531431198 @default.
• W2076142444 cites W1562084799 @default.
• W2076142444 cites W1563035509 @default.
• W2076142444 cites W1563813749 @default.
• W2076142444 cites W1582929466 @default.
• W2076142444 cites W1584902893 @default.
• W2076142444 cites W1592138426 @default.
• W2076142444 cites W1592413740 @default.
• W2076142444 cites W1597723698 @default.
• W2076142444 cites W1603120363 @default.
• W2076142444 cites W1624020394 @default.
• W2076142444 cites W1700388615 @default.
• W2076142444 cites W1767192041 @default.
• W2076142444 cites W1795007707 @default.
• W2076142444 cites W1866615502 @default.
• W2076142444 cites W1890282385 @default.
• W2076142444 cites W1896773931 @default.
• W2076142444 cites W1971048984 @default.
• W2076142444 cites W1975790879 @default.
• W2076142444 cites W1976463089 @default.
• W2076142444 cites W1981232584 @default.
• W2076142444 cites W1989573207 @default.
• W2076142444 cites W1994772282 @default.
• W2076142444 cites W1995403172 @default.
• W2076142444 cites W1996848157 @default.
• W2076142444 cites W2008317513 @default.
• W2076142444 cites W2011603959 @default.
• W2076142444 cites W2012030993 @default.
• W2076142444 cites W2013839625 @default.
• W2076142444 cites W2018289835 @default.
• W2076142444 cites W2021506119 @default.
• W2076142444 cites W2028137817 @default.
• W2076142444 cites W2037123658 @default.
• W2076142444 cites W2059120868 @default.
• W2076142444 cites W2059192413 @default.
• W2076142444 cites W2063572572 @default.
• W2076142444 cites W2064754325 @default.
• W2076142444 cites W2068670552 @default.
• W2076142444 cites W207028343 @default.
• W2076142444 cites W2070947895 @default.
• W2076142444 cites W2073925818 @default.
• W2076142444 cites W2076816515 @default.
• W2076142444 cites W2086263410 @default.
• W2076142444 cites W2094296787 @default.
• W2076142444 cites W2097320436 @default.
• W2076142444 cites W2100837269 @default.
• W2076142444 cites W2102796334 @default.
• W2076142444 cites W2104688697 @default.
• W2076142444 cites W2111341106 @default.
• W2076142444 cites W2119125561 @default.
• W2076142444 cites W2133388060 @default.
• W2076142444 cites W2142750148 @default.
• W2076142444 cites W2146585577 @default.
• W2076142444 cites W2147202849 @default.
• W2076142444 cites W2153172339 @default.
• W2076142444 cites W2175428614 @default.
• W2076142444 doi "https://doi.org/10.1091/mbc.7.4.595" @default.
• W2076142444 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/275912" @default.
• W2076142444 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8730102" @default.
• W2076142444 hasPublicationYear "1996" @default.
• W2076142444 type Work @default.
• W2076142444 sameAs 2076142444 @default.
• W2076142444 citedByCount "19" @default.
• W2076142444 countsByYear W20761424442013 @default.
• W2076142444 countsByYear W20761424442016 @default.
• W2076142444 countsByYear W20761424442018 @default.
• W2076142444 crossrefType "journal-article" @default.
• W2076142444 hasAuthorship W2076142444A5002695228 @default.
• W2076142444 hasAuthorship W2076142444A5067631894 @default.
• W2076142444 hasBestOaLocation W20761424442 @default.
• W2076142444 hasConcept C104317684 @default.
• W2076142444 hasConcept C10858879 @default.
• W2076142444 hasConcept C136961756 @default.
• W2076142444 hasConcept C153911025 @default.
• W2076142444 hasConcept C166703698 @default.
• W2076142444 hasConcept C167625842 @default.
• W2076142444 hasConcept C170493617 @default.
• W2076142444 hasConcept C181199279 @default.
• W2076142444 hasConcept C24530287 @default.
• W2076142444 hasConcept C2778816929 @default.
• W2076142444 hasConcept C55493867 @default.
• W2076142444 hasConcept C67705224 @default.
• W2076142444 hasConcept C86803240 @default.
• W2076142444 hasConcept C95444343 @default.
• W2076142444 hasConceptScore W2076142444C104317684 @default.
• W2076142444 hasConceptScore W2076142444C10858879 @default.
• W2076142444 hasConceptScore W2076142444C136961756 @default.
• W2076142444 hasConceptScore W2076142444C153911025 @default.
• W2076142444 hasConceptScore W2076142444C166703698 @default.

• next