FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076148268> ?p ?o ?g. }

• W2076148268 endingPage "1890" @default.
• W2076148268 startingPage "1888" @default.
• W2076148268 abstract "The diagnosis of toxoplasmic encephalitis (TE) in AIDS patients is mainly based on clinical and radiological features, and on their resolution after specific treatment [1]. There is no optimal laboratory method with high sensitivity and specificity available for this diagnosis. Therefore, a multicentre prospective study was performed in France from June 1993 to September 1994 to assess the value of the detection of Toxoplasma gondii parasitaemia by in vitro culture and polymerase chain reaction (PCR) in HIV-infected patients empirically treated for a first episode of suspected TE. This study enrolled 186 AIDS patients from 21 clinical centres. First, 110 patients were included, and after an intermediate analysis a second series of 76 patients was included. Identification of TE and non-TE cases was determined by an independent validation committee at the completion of the first 6 weeks of therapy; the clinical description of these patients has been reported previously [2]. Ten parasitology laboratories, associated with the 21 clinical centres, performed blood culture and PCR sampling. Blood samples (10 ml) were taken in each patient before starting anti-T. gondii therapy. The in vitro cultures were performed in each laboratory using a previously described standardized technique [3]. Each laboratory divided white cell pellets into aliquots in two separate tubes of equivalent volume that were maintained frozen at −20°C. After the completion of inclusion of each of the two series of patients, the two aliquots of each sample were simultaneously sent, in dry ice, to each of the two laboratories performing the PCR techniques. These two laboratories had important experience in the field of PCR detection of T. gondii [4–10]. Three different PCR techniques were used. For technique A, amplifications were performed as previously described by using a 301 base-pair sequence of the B1 gene of T. gondil [8] and an internal control plasmid DNA. For techniques B and C, amplifications were performed using two different sets of primers [6,7,9], one amplifying a 131 base-pair sequence from the B1 gene (technique B), and the other a 191 base-pair sequence from the target sequence TGR1E of T. gondil (technique C). In both laboratories, carryover contamination was prevented using the Uracil-DNA Glycosylase System (Perkin Elmer, Saint Quentin en Yvelines, France). The results obtained by blood culture and the different PCR techniques are shown in Table 1. Overall, culture was positive in seven out of 113 TE cases and in none of the non-TE cases. According to the technique, positive blood PCR results were obtained in 24.3–29.5% of the TE patients, and in 17.1–25% of the non-TE cases. Results obtained with technique A, and techniques B and C, were statistically independent.Table 1: . Polymerase chain reaction (PCR) results for Toxoplasma gondii DNA detection in blood samples of patients with toxoplasmic encephalitis (TE) and patients in which the diagnosis of TE was excluded (non-TE).In this study, PCR and blood culture for T. gondii detection did not appear to be useful in helping the clinician to initiate specific therapy in AIDS patients with suspected TE. The global sensitivity of PCR was much higher than that of in vitro culture, but remained low compared with the results of the validation committee assessment of TE. This low rate of positivity raises the question of the role of parasitaemia in the pathophysiology of cerebral toxoplasmosis [11]. Since a large percentage of patients in the study were seropositive for T. gondii (87%) [2], it is possible that many patients designated as non-TE may still have had parasitaemia (resulting in a PCR-positive result). However, the most serious problem in our study was the discrepancy between the three techniques and between the first and the second series, despite the fact that the sensitivity of each PCR assay had been assessed using mice blood spiked with defined numbers of parasites. Such discrepancies have been encountered in a previous multicentre study on Mycobacterium tuberculosis [12] and could be due to a low reproducibility of sample pretreatment, unequal distribution of the samples, technical difficulties in isolating white blood cells, contamination from in vitro cultures present in the same room, and potential degradation of low DNA amounts during storage or shipment, for example [13]. However, the results obtained on blood samples that were positive in cell culture demonstrated that when the total DNA amount in a sample was sufficient the results of the different PCR assays were concordant. In conclusion, the results of our study must be interpreted very carefully, since both pathophysiological and technical parameters may have interfered with the results of these sensitive methods. PCR performed on blood samples was not useful for the management of cases of suspected cerebral toxoplasmosis. However, it could remain useful for the diagnosis of extracerebral toxoplasmosis in AIDS patients, for which the clinical and radiological symptoms are more difficult to interpret [4,9,14–16]. Acknowledgements The authors thank the patients and institutions who participated in the development, implementation, or analysis of the Bio-Toxo study and particularly V. Reliquet, A. Huart, C. Leport, J. Weiss and P. Ambroise-Thomas. The authors are also indebted to T. Ouatas and J. Simon for the technical performance of the PCR assays." @default.
• W2076148268 created "2016-06-24" @default.
• W2076148268 creator A5030384136 @default.
• W2076148268 creator A5037466792 @default.
• W2076148268 creator A5050004814 @default.
• W2076148268 creator A5054315981 @default.
• W2076148268 creator A5056512475 @default.
• W2076148268 date "1997-12-01" @default.
• W2076148268 modified "2023-09-24" @default.
• W2076148268 title "A multicentre prospective study for the polymerase chain reaction detection of Toxoplasma gondii DNA in blood samples from 186 AIDS patients with suspected toxoplasmic encephalitis" @default.
• W2076148268 cites W1577369130 @default.
• W2076148268 cites W1763546692 @default.
• W2076148268 cites W1897879351 @default.
• W2076148268 cites W1950784241 @default.
• W2076148268 cites W1966766102 @default.
• W2076148268 cites W1987793349 @default.
• W2076148268 cites W2053966044 @default.
• W2076148268 cites W2065654101 @default.
• W2076148268 cites W2073480594 @default.
• W2076148268 cites W2148602895 @default.
• W2076148268 cites W2165564896 @default.
• W2076148268 cites W2419933799 @default.
• W2076148268 cites W3025190738 @default.
• W2076148268 doi "https://doi.org/10.1097/00002030-199715000-00018" @default.
• W2076148268 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9412712" @default.
• W2076148268 hasPublicationYear "1997" @default.
• W2076148268 type Work @default.
• W2076148268 sameAs 2076148268 @default.
• W2076148268 citedByCount "26" @default.
• W2076148268 countsByYear W20761482682012 @default.
• W2076148268 countsByYear W20761482682014 @default.
• W2076148268 countsByYear W20761482682015 @default.
• W2076148268 countsByYear W20761482682016 @default.
• W2076148268 countsByYear W20761482682019 @default.
• W2076148268 countsByYear W20761482682021 @default.
• W2076148268 countsByYear W20761482682022 @default.
• W2076148268 crossrefType "journal-article" @default.
• W2076148268 hasAuthorship W2076148268A5030384136 @default.
• W2076148268 hasAuthorship W2076148268A5037466792 @default.
• W2076148268 hasAuthorship W2076148268A5050004814 @default.
• W2076148268 hasAuthorship W2076148268A5054315981 @default.
• W2076148268 hasAuthorship W2076148268A5056512475 @default.
• W2076148268 hasBestOaLocation W20761482681 @default.
• W2076148268 hasConcept C104317684 @default.
• W2076148268 hasConcept C126322002 @default.
• W2076148268 hasConcept C141071460 @default.
• W2076148268 hasConcept C142724271 @default.
• W2076148268 hasConcept C159654299 @default.
• W2076148268 hasConcept C188816634 @default.
• W2076148268 hasConcept C203014093 @default.
• W2076148268 hasConcept C2778062946 @default.
• W2076148268 hasConcept C2778128430 @default.
• W2076148268 hasConcept C2778488018 @default.
• W2076148268 hasConcept C49105822 @default.
• W2076148268 hasConcept C55493867 @default.
• W2076148268 hasConcept C71924100 @default.
• W2076148268 hasConcept C86803240 @default.
• W2076148268 hasConcept C90924648 @default.
• W2076148268 hasConceptScore W2076148268C104317684 @default.
• W2076148268 hasConceptScore W2076148268C126322002 @default.
• W2076148268 hasConceptScore W2076148268C141071460 @default.
• W2076148268 hasConceptScore W2076148268C142724271 @default.
• W2076148268 hasConceptScore W2076148268C159654299 @default.
• W2076148268 hasConceptScore W2076148268C188816634 @default.
• W2076148268 hasConceptScore W2076148268C203014093 @default.
• W2076148268 hasConceptScore W2076148268C2778062946 @default.
• W2076148268 hasConceptScore W2076148268C2778128430 @default.
• W2076148268 hasConceptScore W2076148268C2778488018 @default.
• W2076148268 hasConceptScore W2076148268C49105822 @default.
• W2076148268 hasConceptScore W2076148268C55493867 @default.
• W2076148268 hasConceptScore W2076148268C71924100 @default.
• W2076148268 hasConceptScore W2076148268C86803240 @default.
• W2076148268 hasConceptScore W2076148268C90924648 @default.
• W2076148268 hasIssue "15" @default.
• W2076148268 hasLocation W20761482681 @default.
• W2076148268 hasLocation W20761482682 @default.
• W2076148268 hasLocation W20761482683 @default.
• W2076148268 hasOpenAccess W2076148268 @default.
• W2076148268 hasPrimaryLocation W20761482681 @default.
• W2076148268 hasRelatedWork W1967728126 @default.
• W2076148268 hasRelatedWork W1999933111 @default.
• W2076148268 hasRelatedWork W2029232447 @default.
• W2076148268 hasRelatedWork W2082317779 @default.
• W2076148268 hasRelatedWork W2172069200 @default.
• W2076148268 hasRelatedWork W2331976407 @default.
• W2076148268 hasRelatedWork W2368808942 @default.
• W2076148268 hasRelatedWork W2439543579 @default.
• W2076148268 hasRelatedWork W87054969 @default.
• W2076148268 hasRelatedWork W2179426006 @default.
• W2076148268 hasVolume "11" @default.
• W2076148268 isParatext "false" @default.
• W2076148268 isRetracted "false" @default.
• W2076148268 magId "2076148268" @default.
• W2076148268 workType "article" @default.