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- W2076157583 abstract "We describe a reliable method for the simultaneous determination of isotopic enrichments of [1- 13 C]homocysteine, [1- 13 C]methionine and [ 2 H 3 -methyl-1- 13 C]methionine in human plasma. Accurate [1- 13 C]homocysteine calibration standards were prepared by chemical conversion via thiolactonisation of [1- 13 C]methionine standards. Based upon anion-exchange chromatography, (di)acetyl-3,5-bis–trifluoromethylbenzyl derivatives, preparation of accurate calibration curves and gas chromatography–negative chemical ionization mass spectrometry, isotopic enrichments in human plasma could be determined with TTR (%) <±0.2% ( N =3) for [1- 13 C]homocysteine (enrichment range 0–8%), [1- 13 C]methionine (enrichment range 0–3%) and [ 2 H 3 -methyl-1- 13 C]methionine (enrichment range 0–12%). The method was applied in a [ 2 H 3 -methyl-1- 13 C]methionine tracer infusion study in a biological model." @default.
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- W2076157583 date "2000-01-01" @default.
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- W2076157583 title "Determination of isotopic enrichments of [1-13C]homocysteine, [1-13C]methionine and [2H3-methyl-1-13C]methionine in human plasma by gas chromatography–negative chemical ionization mass spectrometry" @default.
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- W2076157583 doi "https://doi.org/10.1016/s0378-4347(99)00504-6" @default.
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