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• W2076172620 endingPage "17777" @default.
• W2076172620 startingPage "17763" @default.
• W2076172620 abstract "Detailed experiments were designed to evaluate the function of load-shock treatment strategy (50 g COD/l; 3 days) for selective enrichment of acidogenic hydrogen (H2) producing consortia in comparison with untreated anaerobic consortia. Experiments performed in suspended-batch mode bioreactors for 520 days illustrated the relative efficiency of load-shock treated consortia in enhancing H2 production (16.64 mol/kg CODR) compared to untreated-parent consortia (3.31 mol/kg CODR). On the contrary, substrate degradation was higher with control operation (ξCOD, 62.86%; substrate degradation rate (SDR), 1.10 kg CODR/m3-day) compared to load-shock culture (52.33%; 0.78 kg CODR/m3-day). Fatty acid composition documented a shift in the metabolic pathway towards acetate formation after applying load-shock, which manifests higher H2 production. Microbial profiling documented a significant alteration in species composition of microbial communities after repeated load-shock applications specific to enrichment of Firmicutes which are favourable for H2 production. Dehydrogenase activity was stabilized with each re-treatment, signifying the adaptation inclination of the biocatalyst towards increased proton shuttling between metabolic intermediates, leading to higher H2 production. Voltammograms of load-shock treated cultures showed a marked shift in oxidation and reduction catalytic currents towards more positive and negative values respectively with increasing scan rate evidencing simultaneous redox-conversion reactions, facilitating proton gradient in the cell towards increased H2 production. Load-shock treatment facilitates direct cultivation of inoculums at higher substrate load without any chemical pretreatment. This study documented the feasibility of controlling microbial metabolic function by application of load-shock treatment either for preparing inoculum for startup of the reactor or to the reactor resident microflora (in situ) during operation whenever required to regain the process performance." @default.
• W2076172620 created "2016-06-24" @default.
• W2076172620 creator A5064632607 @default.
• W2076172620 creator A5090202426 @default.
• W2076172620 date "2012-12-01" @default.
• W2076172620 modified "2023-09-27" @default.
• W2076172620 title "Regulating biohydrogen production from wastewater by applying organic load-shock: Change in the microbial community structure and bio-electrochemical behavior over long-term operation" @default.
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• W2076172620 doi "https://doi.org/10.1016/j.ijhydene.2012.08.124" @default.
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