FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076179963> ?p ?o ?g. }

• W2076179963 endingPage "87" @default.
• W2076179963 startingPage "77" @default.
• W2076179963 abstract "Abstract The Gram-positive bacterium Rhodococcus opacus DSM 43250 produces an l -amino acid oxidase ( l -AAO) with a very broad substrate specificity. This enzyme has been purified to homogeneity and a detailed biochemical characterization was carried out. The complete nucleotide sequence of the l -AAO gene was determined and the primary structure of l -AAO was deduced. The molecular mass of the native enzyme was 99 kDa determined by gel filtration, 54.2/108.5 kDa measured by MALDI-TOF/MS, 53.2 kDa for the subunit calculated after SDS/PAGE. The coenzyme-binding motif G–X–G–X–X–G which is known for all l -AAOs was found very close to the N-terminus of the protein. l -AAO oxidized 39 out of 43 tested l -amino acids. The kinetic data for 16 of these l -amino acids were determined revealing Km-values in the range of 15–30 μM for substrates like l -phenylalanine, l -leucine, l -citrulline and l -lysine. The stability of l -AAO can be increased by storage or incubation of the enzyme in glycine/NaOH buffer. The protein has a pI of 4.8 and a slightly basic pH-optimum at pH 8–9 measured for l -alanine, l -phenylalanine and l -leucine as substrates. The ability for resolution of racemic mixtures was investigated and d -amino acids with an enantiomeric excess of >99% were obtained." @default.
• W2076179963 created "2016-06-24" @default.
• W2076179963 creator A5043185127 @default.
• W2076179963 creator A5045867674 @default.
• W2076179963 date "2002-07-01" @default.
• W2076179963 modified "2023-09-23" @default.
• W2076179963 title "A new bacterial l-amino acid oxidase with a broad substrate specificity: purification and characterization" @default.
• W2076179963 cites W1507887986 @default.
• W2076179963 cites W1515182926 @default.
• W2076179963 cites W1537473069 @default.
• W2076179963 cites W1547009581 @default.
• W2076179963 cites W1608590586 @default.
• W2076179963 cites W1685671312 @default.
• W2076179963 cites W1858904855 @default.
• W2076179963 cites W1948485883 @default.
• W2076179963 cites W1973159471 @default.
• W2076179963 cites W1977822177 @default.
• W2076179963 cites W1982695252 @default.
• W2076179963 cites W1988600174 @default.
• W2076179963 cites W2005211587 @default.
• W2076179963 cites W2016299293 @default.
• W2076179963 cites W2018924194 @default.
• W2076179963 cites W2024682831 @default.
• W2076179963 cites W2027376251 @default.
• W2076179963 cites W2027493599 @default.
• W2076179963 cites W2032974293 @default.
• W2076179963 cites W2033924510 @default.
• W2076179963 cites W2034250833 @default.
• W2076179963 cites W2037384383 @default.
• W2076179963 cites W2053043838 @default.
• W2076179963 cites W2053925771 @default.
• W2076179963 cites W2054492447 @default.
• W2076179963 cites W2055545946 @default.
• W2076179963 cites W2057642401 @default.
• W2076179963 cites W2061927713 @default.
• W2076179963 cites W2068045395 @default.
• W2076179963 cites W2071320462 @default.
• W2076179963 cites W2071412922 @default.
• W2076179963 cites W2074279395 @default.
• W2076179963 cites W2082727072 @default.
• W2076179963 cites W2087118708 @default.
• W2076179963 cites W2092421607 @default.
• W2076179963 cites W2118404178 @default.
• W2076179963 cites W2124740055 @default.
• W2076179963 cites W2163367871 @default.
• W2076179963 cites W2165802825 @default.
• W2076179963 cites W2166374964 @default.
• W2076179963 cites W2323990428 @default.
• W2076179963 cites W4255316968 @default.
• W2076179963 cites W4293247451 @default.
• W2076179963 doi "https://doi.org/10.1016/s0141-0229(02)00072-8" @default.
• W2076179963 hasPublicationYear "2002" @default.
• W2076179963 type Work @default.
• W2076179963 sameAs 2076179963 @default.
• W2076179963 citedByCount "104" @default.
• W2076179963 countsByYear W20761799632012 @default.
• W2076179963 countsByYear W20761799632013 @default.
• W2076179963 countsByYear W20761799632014 @default.
• W2076179963 countsByYear W20761799632015 @default.
• W2076179963 countsByYear W20761799632016 @default.
• W2076179963 countsByYear W20761799632017 @default.
• W2076179963 countsByYear W20761799632018 @default.
• W2076179963 countsByYear W20761799632019 @default.
• W2076179963 countsByYear W20761799632020 @default.
• W2076179963 countsByYear W20761799632021 @default.
• W2076179963 countsByYear W20761799632022 @default.
• W2076179963 countsByYear W20761799632023 @default.
• W2076179963 crossrefType "journal-article" @default.
• W2076179963 hasAuthorship W2076179963A5043185127 @default.
• W2076179963 hasAuthorship W2076179963A5045867674 @default.
• W2076179963 hasConcept C181199279 @default.
• W2076179963 hasConcept C185592680 @default.
• W2076179963 hasConcept C18903297 @default.
• W2076179963 hasConcept C2777289219 @default.
• W2076179963 hasConcept C2994592520 @default.
• W2076179963 hasConcept C55493867 @default.
• W2076179963 hasConcept C71924100 @default.
• W2076179963 hasConcept C86803240 @default.
• W2076179963 hasConcept C89423630 @default.
• W2076179963 hasConceptScore W2076179963C181199279 @default.
• W2076179963 hasConceptScore W2076179963C185592680 @default.
• W2076179963 hasConceptScore W2076179963C18903297 @default.
• W2076179963 hasConceptScore W2076179963C2777289219 @default.
• W2076179963 hasConceptScore W2076179963C2994592520 @default.
• W2076179963 hasConceptScore W2076179963C55493867 @default.
• W2076179963 hasConceptScore W2076179963C71924100 @default.
• W2076179963 hasConceptScore W2076179963C86803240 @default.
• W2076179963 hasConceptScore W2076179963C89423630 @default.
• W2076179963 hasIssue "1-2" @default.
• W2076179963 hasLocation W20761799631 @default.
• W2076179963 hasOpenAccess W2076179963 @default.
• W2076179963 hasPrimaryLocation W20761799631 @default.
• W2076179963 hasRelatedWork W2114659756 @default.
• W2076179963 hasRelatedWork W2136624548 @default.
• W2076179963 hasRelatedWork W2323334504 @default.
• W2076179963 hasRelatedWork W2343755235 @default.
• W2076179963 hasRelatedWork W2398002728 @default.
• W2076179963 hasRelatedWork W2414971833 @default.

• next