FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076190097> ?p ?o ?g. }

• W2076190097 endingPage "257" @default.
• W2076190097 startingPage "257" @default.
• W2076190097 abstract "Today's pork production is very dependent on reproductive efficiency. Any improvements in the production capability (e.g., number of sperm produced) of the animals involved would be invaluable. Many researchers have examined methods to improve oocyte production, but have not focused on the concentration of sperm produced from a single boar used for artificial insemination (AI). The benefit of using AI is that a greater number of females can be bred to a single boar; thus the total amount of sperm per ejaculation is the main factor in the efficiency of AI. An increase in the number of Sertoli cells leads to an increase in testis size and the number of sperm produced because there are a finite number of germ cells that can be supported by the Sertoli cell during spermatogenesis. Therefore, by examining the factors that control the growth and differentiation of Sertoli cells, the amount of sperm per ejaculation in an individual boar can be increased. The purpose of this research is to begin a systematic analysis of cell cycle regulators expressed in the Sertoli cell during testicular development in the pig. By using pigs of different ages, we will establish a baseline of what regulatory factors are present at different time points in the developing Sertoli cell. Identification of certain factors could lead to an increase in sperm production by enabling the growth of Sertoli cells or inhibition of the natural reduction of these cells. Immunohistochemistry (IHC) analysis was performed on testis tissues from different males (n = 2) at various ages (3, 7, 14, 25, and 50 days) prior to puberty. Tissues were fixed in modified Davidson's fixative, embedded in paraffin, and sectioned; slides were processed for IHC. After antigen retrieval, endogenous hydrogen peroxidase was blocked with 0.6% H2O2 and sections were incubated overnight at 4°C with the appropriate antibody. The following antibodies were used to examine the factors controlling Sertoli cell proliferation: GATA-4 (transcription factor specific for developing and adult pig Sertoli cells), Ki67 (a nuclear protein present in all phases of the cell cycle except G0), cyclin-dependent kinase inhibitor p27 (Kip1), and steroid receptors AR (androgen receptor) and LH2 (estrogen receptor). The slides were then incubated with an appropriate secondary antibody, visualized with DAB chromagen, and counterstained with Mayer's hematoxylin. Immunostaining using p27 (Kip1) revealed no positive staining in any of the days tested, as there is cell division during all of these time points. Protein expression for Ki67 stained mildly after Day 25, suggesting that Sertoli cells became more active at this stage of development. The AR weakly stained and GATA-1 stained intensely at all time points. The data for LH2 were inconclusive and the procedure needs to be performed again. These experiments only give a glimpse of the regulatory factors involved in Sertoli cell proliferation in the developing boar testis. Further studies are being planned to explore other potential factors involved." @default.
• W2076190097 created "2016-06-24" @default.
• W2076190097 creator A5002240092 @default.
• W2076190097 creator A5009194998 @default.
• W2076190097 creator A5016594793 @default.
• W2076190097 creator A5058370445 @default.
• W2076190097 creator A5068782130 @default.
• W2076190097 creator A5073984522 @default.
• W2076190097 date "2006-01-01" @default.
• W2076190097 modified "2023-10-17" @default.
• W2076190097 title "300 IMMUNO-EXPRESSION OF POTENTIAL FACTORS REGULATING SERTOLI CELL PROLIFERATION IN THE PREPUBERTAL BOAR" @default.
• W2076190097 doi "https://doi.org/10.1071/rdv18n2ab300" @default.
• W2076190097 hasPublicationYear "2006" @default.
• W2076190097 type Work @default.
• W2076190097 sameAs 2076190097 @default.
• W2076190097 citedByCount "0" @default.
• W2076190097 crossrefType "journal-article" @default.
• W2076190097 hasAuthorship W2076190097A5002240092 @default.
• W2076190097 hasAuthorship W2076190097A5009194998 @default.
• W2076190097 hasAuthorship W2076190097A5016594793 @default.
• W2076190097 hasAuthorship W2076190097A5058370445 @default.
• W2076190097 hasAuthorship W2076190097A5068782130 @default.
• W2076190097 hasAuthorship W2076190097A5073984522 @default.
• W2076190097 hasConcept C104317684 @default.
• W2076190097 hasConcept C123765429 @default.
• W2076190097 hasConcept C126322002 @default.
• W2076190097 hasConcept C134018914 @default.
• W2076190097 hasConcept C16685009 @default.
• W2076190097 hasConcept C2777005246 @default.
• W2076190097 hasConcept C2777359717 @default.
• W2076190097 hasConcept C2777812360 @default.
• W2076190097 hasConcept C2779122487 @default.
• W2076190097 hasConcept C2781068068 @default.
• W2076190097 hasConcept C2781087480 @default.
• W2076190097 hasConcept C54355233 @default.
• W2076190097 hasConcept C71924100 @default.
• W2076190097 hasConcept C86803240 @default.
• W2076190097 hasConceptScore W2076190097C104317684 @default.
• W2076190097 hasConceptScore W2076190097C123765429 @default.
• W2076190097 hasConceptScore W2076190097C126322002 @default.
• W2076190097 hasConceptScore W2076190097C134018914 @default.
• W2076190097 hasConceptScore W2076190097C16685009 @default.
• W2076190097 hasConceptScore W2076190097C2777005246 @default.
• W2076190097 hasConceptScore W2076190097C2777359717 @default.
• W2076190097 hasConceptScore W2076190097C2777812360 @default.
• W2076190097 hasConceptScore W2076190097C2779122487 @default.
• W2076190097 hasConceptScore W2076190097C2781068068 @default.
• W2076190097 hasConceptScore W2076190097C2781087480 @default.
• W2076190097 hasConceptScore W2076190097C54355233 @default.
• W2076190097 hasConceptScore W2076190097C71924100 @default.
• W2076190097 hasConceptScore W2076190097C86803240 @default.
• W2076190097 hasIssue "2" @default.
• W2076190097 hasLocation W20761900971 @default.
• W2076190097 hasOpenAccess W2076190097 @default.
• W2076190097 hasPrimaryLocation W20761900971 @default.
• W2076190097 hasRelatedWork W1479700143 @default.
• W2076190097 hasRelatedWork W1498186062 @default.
• W2076190097 hasRelatedWork W2017473812 @default.
• W2076190097 hasRelatedWork W2075276287 @default.
• W2076190097 hasRelatedWork W2076190097 @default.
• W2076190097 hasRelatedWork W2156321367 @default.
• W2076190097 hasRelatedWork W2479155331 @default.
• W2076190097 hasRelatedWork W2729223853 @default.
• W2076190097 hasRelatedWork W3043398618 @default.
• W2076190097 hasRelatedWork W1499076273 @default.
• W2076190097 hasVolume "18" @default.
• W2076190097 isParatext "false" @default.
• W2076190097 isRetracted "false" @default.
• W2076190097 magId "2076190097" @default.
• W2076190097 workType "article" @default.