FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076201212> ?p ?o ?g. }

• W2076201212 endingPage "1184" @default.
• W2076201212 startingPage "1178" @default.
• W2076201212 abstract "In allergic inflammation and asthma, eosinophils are major effector cells. They have been shown to synthesize at least 23 cytokines, some of which are stored intracellularly in their unique crystalloid granules together with cationic granule protein. Little is known about the synthesis and storage of cytokines relative to cationic granule proteins in maturing eosinophils during eosinophilopoiesis.Our purpose was to analyze the expression of eosinophil-derived mediators, major basic protein (MBP), eosinophil cationic protein (ECP), IL-6, and RANTES, during early stages of eosinophil maturation in CD34(+) cell-derived colonies.Purified human cord blood CD34(+) cells were grown in methylcellulose cultures in the presence of recombinant human IL-3 and IL-5. By confocal laser scanning microscopy, the coexpression of eosinophil granular proteins MBP and ECP was determined concurrently with IL-6 and RANTES during eosinophil maturation on days 16, 19, 23, and 28 of culture.Immunoreactivity against MBP, ECP, IL-6, and RANTES was not detectable in freshly purified CD34(+) cells. Maturing eosinophils (>95%) exhibited positive immunostaining for all these proteins between days 16 and 28 of culture. At early stages of culture, discrete immunostaining was observed around the periphery but not in the center of granular structures. By day 28 cultured eosinophil-like cells showed evidence of the acquisition of crystalloid granule-like structures, analogous to those observed in mature peripheral blood eosinophils.Eosinophils express and store cytokines simultaneously with cationic granule proteins during the process of maturation. We propose that the storage of cytokines during the development of eosinophils is an early event and it may be integral to inflammatory responses involving these cells. The results of this study suggest a potential immunoregulatory function for maturing eosinophils." @default.
• W2076201212 created "2016-06-24" @default.
• W2076201212 creator A5060383332 @default.
• W2076201212 creator A5064717671 @default.
• W2076201212 creator A5073058599 @default.
• W2076201212 creator A5086948999 @default.
• W2076201212 creator A5090837792 @default.
• W2076201212 date "2000-06-01" @default.
• W2076201212 modified "2023-10-15" @default.
• W2076201212 title "Immunofluorescence analysis of cytokine and granule protein expression during eosinophil maturation from cord blood–derived CD34+ progenitors" @default.
• W2076201212 cites W1482418860 @default.
• W2076201212 cites W1572349468 @default.
• W2076201212 cites W1589957195 @default.
• W2076201212 cites W163520811 @default.
• W2076201212 cites W1840906208 @default.
• W2076201212 cites W1964002710 @default.
• W2076201212 cites W1984324451 @default.
• W2076201212 cites W1990786859 @default.
• W2076201212 cites W2027344845 @default.
• W2076201212 cites W2028004934 @default.
• W2076201212 cites W2028740051 @default.
• W2076201212 cites W2029933879 @default.
• W2076201212 cites W2053082547 @default.
• W2076201212 cites W2066011467 @default.
• W2076201212 cites W2074353240 @default.
• W2076201212 cites W2113995576 @default.
• W2076201212 cites W2134812776 @default.
• W2076201212 cites W2135431323 @default.
• W2076201212 cites W2141367196 @default.
• W2076201212 cites W2154416360 @default.
• W2076201212 cites W2158312939 @default.
• W2076201212 cites W2227609407 @default.
• W2076201212 cites W2297975249 @default.
• W2076201212 cites W2315876050 @default.
• W2076201212 cites W2411229321 @default.
• W2076201212 cites W4248080989 @default.
• W2076201212 doi "https://doi.org/10.1067/mai.2000.106930" @default.
• W2076201212 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10856153" @default.
• W2076201212 hasPublicationYear "2000" @default.
• W2076201212 type Work @default.
• W2076201212 sameAs 2076201212 @default.
• W2076201212 citedByCount "23" @default.
• W2076201212 countsByYear W20762012122012 @default.
• W2076201212 countsByYear W20762012122013 @default.
• W2076201212 countsByYear W20762012122018 @default.
• W2076201212 countsByYear W20762012122023 @default.
• W2076201212 crossrefType "journal-article" @default.
• W2076201212 hasAuthorship W2076201212A5060383332 @default.
• W2076201212 hasAuthorship W2076201212A5064717671 @default.
• W2076201212 hasAuthorship W2076201212A5073058599 @default.
• W2076201212 hasAuthorship W2076201212A5086948999 @default.
• W2076201212 hasAuthorship W2076201212A5090837792 @default.
• W2076201212 hasBestOaLocation W20762012121 @default.
• W2076201212 hasConcept C153911025 @default.
• W2076201212 hasConcept C185592680 @default.
• W2076201212 hasConcept C201750760 @default.
• W2076201212 hasConcept C203014093 @default.
• W2076201212 hasConcept C204232928 @default.
• W2076201212 hasConcept C2776042228 @default.
• W2076201212 hasConcept C2776914184 @default.
• W2076201212 hasConcept C2777037409 @default.
• W2076201212 hasConcept C2777129736 @default.
• W2076201212 hasConcept C2778690821 @default.
• W2076201212 hasConcept C2779675166 @default.
• W2076201212 hasConcept C2780914630 @default.
• W2076201212 hasConcept C28328180 @default.
• W2076201212 hasConcept C2910475272 @default.
• W2076201212 hasConcept C4224716 @default.
• W2076201212 hasConcept C60483475 @default.
• W2076201212 hasConcept C86803240 @default.
• W2076201212 hasConcept C88675164 @default.
• W2076201212 hasConcept C95444343 @default.
• W2076201212 hasConceptScore W2076201212C153911025 @default.
• W2076201212 hasConceptScore W2076201212C185592680 @default.
• W2076201212 hasConceptScore W2076201212C201750760 @default.
• W2076201212 hasConceptScore W2076201212C203014093 @default.
• W2076201212 hasConceptScore W2076201212C204232928 @default.
• W2076201212 hasConceptScore W2076201212C2776042228 @default.
• W2076201212 hasConceptScore W2076201212C2776914184 @default.
• W2076201212 hasConceptScore W2076201212C2777037409 @default.
• W2076201212 hasConceptScore W2076201212C2777129736 @default.
• W2076201212 hasConceptScore W2076201212C2778690821 @default.
• W2076201212 hasConceptScore W2076201212C2779675166 @default.
• W2076201212 hasConceptScore W2076201212C2780914630 @default.
• W2076201212 hasConceptScore W2076201212C28328180 @default.
• W2076201212 hasConceptScore W2076201212C2910475272 @default.
• W2076201212 hasConceptScore W2076201212C4224716 @default.
• W2076201212 hasConceptScore W2076201212C60483475 @default.
• W2076201212 hasConceptScore W2076201212C86803240 @default.
• W2076201212 hasConceptScore W2076201212C88675164 @default.
• W2076201212 hasConceptScore W2076201212C95444343 @default.
• W2076201212 hasIssue "6" @default.
• W2076201212 hasLocation W20762012121 @default.
• W2076201212 hasLocation W20762012122 @default.
• W2076201212 hasOpenAccess W2076201212 @default.
• W2076201212 hasPrimaryLocation W20762012121 @default.
• W2076201212 hasRelatedWork W1215160743 @default.
• W2076201212 hasRelatedWork W2018947618 @default.

• next