FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076203350> ?p ?o ?g. }

• W2076203350 endingPage "176" @default.
• W2076203350 startingPage "169" @default.
• W2076203350 abstract "Although human islet allotransplantation has been shown to be effective in restoring normoglycaemia to diabetic recipients, the long-term success rate is relatively low. The poor results of human islet transplantation are surprising given the relative ease of establishing functioning islet allografts in rodent models with minimal immunosuppression and have been attributed to a number of factors of which rejection may play a major part. The impracticality of studying the immunogenicity of human islet in vivo has led us to develop a model specifically to study the allogenic immune response to the major components of isolated human islet preparations. To try and overcome the variability associated with this methodology, this study was designed to develop and optimize the mixed lymphocyte coculture as an in vitro model for the initial alloresponse to both freshly isolated intact human islets (MLIC) and acinar tissue pieces (MLAC). For this, titration of the islets and acinar tissue, as well as kinetic studies of the response, were used to select optimum conditions for the MLIC which were shown to be ten islets and ten acinar tissue pieces with a coculture duration of 7–9 days. The optimized studies included the use of non-tissue culture microwells to deter fibroblast growth, RPMI + 10% human AB serum to reduce the background lymphocyte response and a low concentration of dithizone to stain the islets prior to handpicking. Freshly isolated human islets were found to stimulate allogeneic lymphocytic proliferation; however, differences in the relative response to islets and acinar tissue led us to test the effect of soluble products from the acinar cells on lymphocyte proliferation in the mixed lymphocyte reaction. An inhibitory effect of the soluble products of acinar cells on the allogeneic lymphocyte proliferative response was found to contribute to the reduced response of the MLAC compared to the MLIC. Human islets were shown to stimulate an allogeneic immune response in vitro , and this MLIC model provides a method for assessing the specific immunogenicity of human isolated islet preparations and associated acinar tissue in transplantation." @default.
• W2076203350 created "2016-06-24" @default.
• W2076203350 creator A5017076090 @default.
• W2076203350 creator A5050910056 @default.
• W2076203350 creator A5077019394 @default.
• W2076203350 creator A5082673689 @default.
• W2076203350 date "1996-06-01" @default.
• W2076203350 modified "2023-10-16" @default.
• W2076203350 title "Development and optimization of the human allogeneic mixed lymphocyte islet (MLIC) and acinar (MLAC) coculture system" @default.
• W2076203350 cites W143217977 @default.
• W2076203350 cites W1970042981 @default.
• W2076203350 cites W1982505026 @default.
• W2076203350 cites W1983348978 @default.
• W2076203350 cites W1987480361 @default.
• W2076203350 cites W1992837123 @default.
• W2076203350 cites W2006815964 @default.
• W2076203350 cites W2010215926 @default.
• W2076203350 cites W2023211743 @default.
• W2076203350 cites W2025377941 @default.
• W2076203350 cites W2045341864 @default.
• W2076203350 cites W2075952835 @default.
• W2076203350 cites W2076844007 @default.
• W2076203350 cites W2124495648 @default.
• W2076203350 cites W2136224984 @default.
• W2076203350 cites W2149036744 @default.
• W2076203350 cites W2149138664 @default.
• W2076203350 cites W2408893474 @default.
• W2076203350 cites W2412501478 @default.
• W2076203350 cites W2433636140 @default.
• W2076203350 cites W2436901502 @default.
• W2076203350 cites W2471079225 @default.
• W2076203350 cites W2474461208 @default.
• W2076203350 cites W2769264260 @default.
• W2076203350 cites W600594209 @default.
• W2076203350 cites W2414248883 @default.
• W2076203350 doi "https://doi.org/10.1016/s0966-3274(96)80012-8" @default.
• W2076203350 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8843595" @default.
• W2076203350 hasPublicationYear "1996" @default.
• W2076203350 type Work @default.
• W2076203350 sameAs 2076203350 @default.
• W2076203350 citedByCount "10" @default.
• W2076203350 countsByYear W20762033502017 @default.
• W2076203350 countsByYear W20762033502020 @default.
• W2076203350 countsByYear W20762033502021 @default.
• W2076203350 crossrefType "journal-article" @default.
• W2076203350 hasAuthorship W2076203350A5017076090 @default.
• W2076203350 hasAuthorship W2076203350A5050910056 @default.
• W2076203350 hasAuthorship W2076203350A5077019394 @default.
• W2076203350 hasAuthorship W2076203350A5082673689 @default.
• W2076203350 hasConcept C126322002 @default.
• W2076203350 hasConcept C134018914 @default.
• W2076203350 hasConcept C165220095 @default.
• W2076203350 hasConcept C16685009 @default.
• W2076203350 hasConcept C202751555 @default.
• W2076203350 hasConcept C203014093 @default.
• W2076203350 hasConcept C207001950 @default.
• W2076203350 hasConcept C2777561873 @default.
• W2076203350 hasConcept C2777761686 @default.
• W2076203350 hasConcept C2780252810 @default.
• W2076203350 hasConcept C2780868878 @default.
• W2076203350 hasConcept C2911091166 @default.
• W2076203350 hasConcept C54355233 @default.
• W2076203350 hasConcept C55493867 @default.
• W2076203350 hasConcept C555293320 @default.
• W2076203350 hasConcept C71924100 @default.
• W2076203350 hasConcept C86803240 @default.
• W2076203350 hasConcept C8891405 @default.
• W2076203350 hasConceptScore W2076203350C126322002 @default.
• W2076203350 hasConceptScore W2076203350C134018914 @default.
• W2076203350 hasConceptScore W2076203350C165220095 @default.
• W2076203350 hasConceptScore W2076203350C16685009 @default.
• W2076203350 hasConceptScore W2076203350C202751555 @default.
• W2076203350 hasConceptScore W2076203350C203014093 @default.
• W2076203350 hasConceptScore W2076203350C207001950 @default.
• W2076203350 hasConceptScore W2076203350C2777561873 @default.
• W2076203350 hasConceptScore W2076203350C2777761686 @default.
• W2076203350 hasConceptScore W2076203350C2780252810 @default.
• W2076203350 hasConceptScore W2076203350C2780868878 @default.
• W2076203350 hasConceptScore W2076203350C2911091166 @default.
• W2076203350 hasConceptScore W2076203350C54355233 @default.
• W2076203350 hasConceptScore W2076203350C55493867 @default.
• W2076203350 hasConceptScore W2076203350C555293320 @default.
• W2076203350 hasConceptScore W2076203350C71924100 @default.
• W2076203350 hasConceptScore W2076203350C86803240 @default.
• W2076203350 hasConceptScore W2076203350C8891405 @default.
• W2076203350 hasIssue "2" @default.
• W2076203350 hasLocation W20762033501 @default.
• W2076203350 hasLocation W20762033502 @default.
• W2076203350 hasOpenAccess W2076203350 @default.
• W2076203350 hasPrimaryLocation W20762033501 @default.
• W2076203350 hasRelatedWork W1964150730 @default.
• W2076203350 hasRelatedWork W1974461518 @default.
• W2076203350 hasRelatedWork W1982775664 @default.
• W2076203350 hasRelatedWork W2045244606 @default.
• W2076203350 hasRelatedWork W2084272222 @default.
• W2076203350 hasRelatedWork W2170762237 @default.
• W2076203350 hasRelatedWork W2413008984 @default.
• W2076203350 hasRelatedWork W2427169615 @default.

• next