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- W2076211262 abstract "For the quantification of glutamic acid in Ringer's solution, pentafluoropropionic methyl ester was the most sensitive derivative. The detectable concentration was 0.01 m̈M glutamic acid in Ringer's solution; the amount of the preparation was 1 pmol and the injection into a gas chromatograph mass spectrometer was 10 fmol. For the quantification of m̈-aminobutyric acid in Ringer's solution, the trifluoroacetal-hexafluoropropionyl ester was detectable at a concentration of 0.01 m̈M. Ringer's salts facilitated acylation in the order heptafluorobutyric anhydride > pentafluoropropionic anhydride > trifluoroacetic anhydride. The effect depended on esterification of carboxy groups in the order methyl ester > hexafluoropropionyl ester > butyl ester. Sodium carbonate, sodium acetate and sodium citrate also facilitated acylation with pentafluoropropionic anhydride, while sodium phosphate inhibited the acylation and sodium sulfate inhibited it slightly. The pentafluoropropionic methyl ester of glutamic acid was stable for up to 10 days, when it was dissolved in acetone and stored at–8°C." @default.
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- W2076211262 date "1981-04-01" @default.
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- W2076211262 title "Determination of glutamic acid and ω-amino-butyric acid in Ringer's solution without desalination at the femtomole level by gas chromatography chemical ionization mass spectrometry" @default.
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- W2076211262 doi "https://doi.org/10.1002/bms.1200080407" @default.
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