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- W2076214456 abstract "A novel capillary electrophoretic enzyme immunoassay with electrochemical detection (CE-EIA-ED) was developed for a tumor marker cancer antigen 125 (CA125). In this method, after the noncompetitive enzyme immunoreaction, the free enzyme (horseradish peroxidase)-labeled anti-CA125 antibody (Ab∗) and the bound enzyme-labeled complex (Ag–Ab∗) were separated in a separation capillary and then catalyzed the enzyme substrate (3,3,5,5-tetramethyl-benzidine dihydrochloride, TMB(Red)) and H2O2 in a reaction capillary following the separation capillary. The product of the enzymatic catalysis reaction (TMB(Ox)) was amperometrically detected on a carbon fiber microdisk bundle electrode. A activity concentration limit of detection (LOD) of 0.29 U/ml, which corresponded to a activity LOD of 1.6 μU was achieved. The assay could be used to determine CA125 in human serums from ovary tumor patents." @default.
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- W2076214456 date "2003-11-01" @default.
- W2076214456 modified "2023-10-10" @default.
- W2076214456 title "Determination of tumor marker CA125 by capillary electrophoretic enzyme immunoassay with electrochemical detection" @default.
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- W2076214456 doi "https://doi.org/10.1016/s0003-2670(03)00880-8" @default.
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