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• W2076221091 endingPage "731" @default.
• W2076221091 startingPage "723" @default.
• W2076221091 abstract "The mutant YY in the reaction center of Rhodobacter sphaeroides, in which Phe181 on the L chain has been replaced by Tyr, and the double mutant FY, with Tyr210 on the M chain replaced by Phe and Phe181 on the L chain replaced by Tyr, have been constructed by site-directed mutagenesis. The studies described here were performed to complement a previous mutational analysis of mutant FF with Tyr210 replaced by Phe. Both new strains grow photoheterotrophically. The optical absorption spectra of reaction centers isolated from these mutants have band shifts attributable to the monomer bacteriochlorophylls in the vicinity of the substitutions. Photochemical trapping of the bacteriopheophytin anion (I-) indicates that the bacteriopheophytin on the B branch is reduced to a much greater extent in FF and FY as compared to YY and wild-type YF. Low temperature (77 K) absorption spectra clearly show that in the wild-type (YF) and YY reaction centers only the 545-nm-absorbing bacteriopheophytin is reduced while in the FF and FY reaction centers both the 535-nm and 545-nm-absorbing bacteriopheophytins are reduced. A simple kinetic analysis is used to explain these results. This analysis suggests that, in order for the observed trapping results to occur, a decrease in the 'cycling' time must take place, that is changes in the rate(s) of charge recombination must accompany the already known decrease in the forward electron transfer rate." @default.
• W2076221091 created "2016-06-24" @default.
• W2076221091 creator A5034207603 @default.
• W2076221091 creator A5077404586 @default.
• W2076221091 creator A5088909232 @default.
• W2076221091 date "1992-07-01" @default.
• W2076221091 modified "2023-09-24" @default.
• W2076221091 title "Photochemical trapping of a bacteriopheophytin anion in site-specific reaction-center mutants from the photosynthetic bacterium Rhodobacter sphaeroides" @default.
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• W2076221091 doi "https://doi.org/10.1111/j.1432-1033.1992.tb17102.x" @default.
• W2076221091 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1633823" @default.
• W2076221091 hasPublicationYear "1992" @default.
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