FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076222021> ?p ?o ?g. }

• W2076222021 abstract "Abstract The glucose 6-phosphate (Glc-6-P)-induced solubilization of mitochondrial hexokinase (ATP: d -hexose 6-phosphotransferase, EC 2.7.1.1) from rat brain can be reversed by low concentrations (ionic strength m ) of neutral salts. When compared to the original particulate enzyme (i.e., enzyme found on the particles prior to solubilization by Glc-6-P), the rebound enzyme is similar in distribution on sucrose gradients, K m for ATP, inhibition by antiserum to purified brain hexokinase, and resistance to removal by exhaustive washing of the particles. The effectiveness of chloride salts at promoting rebinding increases in the order Cs + + + ≤ Na + + 2+ . This salt-induced rebinding is attributed to the screening of negative charges on the enzyme and/or membrane by cations, thereby decreasing repulsive forces and enhancing attractive interactions between enzyme and membrane. Solubilization of the enzyme, both in the presence and absence of Glc-6-P, is increased at alkaline pH, as would be expected due to increasing repulsive interactions between negative charges on membrane and enzyme as the pH is increased beyond the pI of the enzyme (pI = 6.3). In contrast to previous interpretations, P i displayed no special efficacy at reversing Glc-6-P-induced solubilization, being comparable to other neutral salts on an ionic strength basis. However, P i and its structural analog, arsenate, were shown to counteract specifically the Glc-6-P-induced inhibition and conformational change in the enzyme. At higher concentrations (ionic strength >~ 0.02 m ) neutral salts themselves lead to reversible dissociation of the enzyme from the mitochondria. The efficacy of the salts depends primarily on the pH and on the position of the anion in the Hofmeister series, with salts of chaotropic anions (SCN − , I − , Br − ) being most effective. At pH 6, both chaotropic and nonchaotropic salts solubilize the enzyme, while at pH 8.5, only the chaotropes retain this ability. Neutral salts also have a reversible effect on the conformation of the enzyme, as reflected by enzymatic activity, with chaotropic salts again being most effective; there is no pronounced influence of pH (in the range of pH 6–8.5) on the ability of the salts to cause conformational change in the enzyme. Based on a lack of correlation between saltinduced solubilization and conformational changes affecting activity, it is concluded that the latter are not directly responsible for release of the enzyme from the membrane. In the presence of KSCN, the extent of solubilization decreased with increase in temperature, indicating a negative enthalpy for solubilization. In contrast, in the absence of salt, the enthalpy for solubilization was positive. These temperature effects and the effects of neutral salts on the hexokinase-membrane interaction are interpreted in terms of a model in which electrostatic forces are considered to be of major importance. At low ionic strength, repulsive forces between negative charges on enzyme and membrane predominate; screening of these charges by cations diminishes the repulsion, effectively enhancing attractive electrostatic forces between enzyme and membrane and thus promoting their interaction. At higher ionic strengths, the attractive electrostatic forces are themselves disrupted, resulting in dissociation of the enzyme from the membrane. It is proposed that the greater effectiveness of chaotropic salts at disrupting these attractive forces is due to their increased ability to penetrate through hydrophobic regions of enzyme and membrane to relatively inaccessible sites of electrostatic-interaction." @default.
• W2076222021 created "2016-06-24" @default.
• W2076222021 creator A5010232221 @default.
• W2076222021 creator A5086479392 @default.
• W2076222021 date "1977-07-01" @default.
• W2076222021 modified "2023-09-27" @default.
• W2076222021 title "Effect of neutral salts on the interaction of rat brain hexokinase with the outer mitochondrial membrane" @default.
• W2076222021 cites W1501282895 @default.
• W2076222021 cites W1530586599 @default.
• W2076222021 cites W1550155885 @default.
• W2076222021 cites W157808733 @default.
• W2076222021 cites W1912085582 @default.
• W2076222021 cites W1970881828 @default.
• W2076222021 cites W1978856605 @default.
• W2076222021 cites W1987402041 @default.
• W2076222021 cites W1987980063 @default.
• W2076222021 cites W2003331874 @default.
• W2076222021 cites W2008960133 @default.
• W2076222021 cites W2009726496 @default.
• W2076222021 cites W2023949146 @default.
• W2076222021 cites W2024025956 @default.
• W2076222021 cites W2034771065 @default.
• W2076222021 cites W2035797259 @default.
• W2076222021 cites W2048609504 @default.
• W2076222021 cites W2055780936 @default.
• W2076222021 cites W2056739157 @default.
• W2076222021 cites W2069935619 @default.
• W2076222021 cites W2073295252 @default.
• W2076222021 cites W2079487567 @default.
• W2076222021 cites W2082284520 @default.
• W2076222021 cites W2086186687 @default.
• W2076222021 cites W2130617281 @default.
• W2076222021 cites W2397861637 @default.
• W2076222021 doi "https://doi.org/10.1016/0003-9861(77)90309-5" @default.
• W2076222021 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18993" @default.
• W2076222021 hasPublicationYear "1977" @default.
• W2076222021 type Work @default.
• W2076222021 sameAs 2076222021 @default.
• W2076222021 citedByCount "61" @default.
• W2076222021 countsByYear W20762220212012 @default.
• W2076222021 countsByYear W20762220212014 @default.
• W2076222021 countsByYear W20762220212016 @default.
• W2076222021 countsByYear W20762220212017 @default.
• W2076222021 crossrefType "journal-article" @default.
• W2076222021 hasAuthorship W2076222021A5010232221 @default.
• W2076222021 hasAuthorship W2076222021A5086479392 @default.
• W2076222021 hasConcept C102931765 @default.
• W2076222021 hasConcept C12554922 @default.
• W2076222021 hasConcept C178790620 @default.
• W2076222021 hasConcept C179104552 @default.
• W2076222021 hasConcept C181199279 @default.
• W2076222021 hasConcept C184651966 @default.
• W2076222021 hasConcept C185592680 @default.
• W2076222021 hasConcept C187428577 @default.
• W2076222021 hasConcept C20251656 @default.
• W2076222021 hasConcept C2776371256 @default.
• W2076222021 hasConcept C2779826832 @default.
• W2076222021 hasConcept C28212737 @default.
• W2076222021 hasConcept C41625074 @default.
• W2076222021 hasConcept C55493867 @default.
• W2076222021 hasConcept C71240020 @default.
• W2076222021 hasConcept C86803240 @default.
• W2076222021 hasConceptScore W2076222021C102931765 @default.
• W2076222021 hasConceptScore W2076222021C12554922 @default.
• W2076222021 hasConceptScore W2076222021C178790620 @default.
• W2076222021 hasConceptScore W2076222021C179104552 @default.
• W2076222021 hasConceptScore W2076222021C181199279 @default.
• W2076222021 hasConceptScore W2076222021C184651966 @default.
• W2076222021 hasConceptScore W2076222021C185592680 @default.
• W2076222021 hasConceptScore W2076222021C187428577 @default.
• W2076222021 hasConceptScore W2076222021C20251656 @default.
• W2076222021 hasConceptScore W2076222021C2776371256 @default.
• W2076222021 hasConceptScore W2076222021C2779826832 @default.
• W2076222021 hasConceptScore W2076222021C28212737 @default.
• W2076222021 hasConceptScore W2076222021C41625074 @default.
• W2076222021 hasConceptScore W2076222021C55493867 @default.
• W2076222021 hasConceptScore W2076222021C71240020 @default.
• W2076222021 hasConceptScore W2076222021C86803240 @default.
• W2076222021 hasLocation W20762220211 @default.
• W2076222021 hasLocation W20762220212 @default.
• W2076222021 hasOpenAccess W2076222021 @default.
• W2076222021 hasPrimaryLocation W20762220211 @default.
• W2076222021 hasRelatedWork W1969877512 @default.
• W2076222021 hasRelatedWork W1990159729 @default.
• W2076222021 hasRelatedWork W1997100257 @default.
• W2076222021 hasRelatedWork W2004000230 @default.
• W2076222021 hasRelatedWork W2017341376 @default.
• W2076222021 hasRelatedWork W2033266444 @default.
• W2076222021 hasRelatedWork W2038880126 @default.
• W2076222021 hasRelatedWork W2058324844 @default.
• W2076222021 hasRelatedWork W2092125227 @default.
• W2076222021 hasRelatedWork W2147865251 @default.
• W2076222021 isParatext "false" @default.
• W2076222021 isRetracted "false" @default.
• W2076222021 magId "2076222021" @default.
• W2076222021 workType "article" @default.