Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076230829> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2076230829 endingPage "6" @default.
- W2076230829 startingPage "1" @default.
- W2076230829 abstract "Immunoglobulin heavy chain Fd gene and κ chain gene were amplified by Polymerase Chain Reaction (PCR) from total RNA of a hybridoma cell line secreting anti-stomach cancer monoclonal antibody 3G9. Sequences analysis showed that VH, D, Jhgenes were derived from VH7183, DFL16. 1, and JH3, and that Vk and Jk from VkII and J.2.3G9 Fd and κ DNA segments were cloned into expression vector pComb3. Soluble Fab was expressed by IPTG induction and proved by western blotting. The specific antigen binding activity of the bacterially produced 3G9 Fab was demonstrated by enzyme- linked immunofiltration assay." @default.
- W2076230829 created "2016-06-24" @default.
- W2076230829 creator A5003802936 @default.
- W2076230829 creator A5011569958 @default.
- W2076230829 creator A5015624405 @default.
- W2076230829 creator A5044367029 @default.
- W2076230829 creator A5091104659 @default.
- W2076230829 date "1993-03-01" @default.
- W2076230829 modified "2023-09-25" @default.
- W2076230829 title "Cloning and expression of Fab genes of anti-stomach cancer McAb 3G9 inE. coli" @default.
- W2076230829 cites W1484092314 @default.
- W2076230829 cites W2018107765 @default.
- W2076230829 cites W2061530347 @default.
- W2076230829 cites W2071378581 @default.
- W2076230829 cites W2134812217 @default.
- W2076230829 doi "https://doi.org/10.1007/bf02997485" @default.
- W2076230829 hasPublicationYear "1993" @default.
- W2076230829 type Work @default.
- W2076230829 sameAs 2076230829 @default.
- W2076230829 citedByCount "0" @default.
- W2076230829 crossrefType "journal-article" @default.
- W2076230829 hasAuthorship W2076230829A5003802936 @default.
- W2076230829 hasAuthorship W2076230829A5011569958 @default.
- W2076230829 hasAuthorship W2076230829A5015624405 @default.
- W2076230829 hasAuthorship W2076230829A5044367029 @default.
- W2076230829 hasAuthorship W2076230829A5091104659 @default.
- W2076230829 hasConcept C104317684 @default.
- W2076230829 hasConcept C121050878 @default.
- W2076230829 hasConcept C133307689 @default.
- W2076230829 hasConcept C153911025 @default.
- W2076230829 hasConcept C159654299 @default.
- W2076230829 hasConcept C185592680 @default.
- W2076230829 hasConcept C199360897 @default.
- W2076230829 hasConcept C36394416 @default.
- W2076230829 hasConcept C41008148 @default.
- W2076230829 hasConcept C49105822 @default.
- W2076230829 hasConcept C542903549 @default.
- W2076230829 hasConcept C54355233 @default.
- W2076230829 hasConcept C552990157 @default.
- W2076230829 hasConcept C55493867 @default.
- W2076230829 hasConcept C85265743 @default.
- W2076230829 hasConcept C86803240 @default.
- W2076230829 hasConceptScore W2076230829C104317684 @default.
- W2076230829 hasConceptScore W2076230829C121050878 @default.
- W2076230829 hasConceptScore W2076230829C133307689 @default.
- W2076230829 hasConceptScore W2076230829C153911025 @default.
- W2076230829 hasConceptScore W2076230829C159654299 @default.
- W2076230829 hasConceptScore W2076230829C185592680 @default.
- W2076230829 hasConceptScore W2076230829C199360897 @default.
- W2076230829 hasConceptScore W2076230829C36394416 @default.
- W2076230829 hasConceptScore W2076230829C41008148 @default.
- W2076230829 hasConceptScore W2076230829C49105822 @default.
- W2076230829 hasConceptScore W2076230829C542903549 @default.
- W2076230829 hasConceptScore W2076230829C54355233 @default.
- W2076230829 hasConceptScore W2076230829C552990157 @default.
- W2076230829 hasConceptScore W2076230829C55493867 @default.
- W2076230829 hasConceptScore W2076230829C85265743 @default.
- W2076230829 hasConceptScore W2076230829C86803240 @default.
- W2076230829 hasIssue "1" @default.
- W2076230829 hasLocation W20762308291 @default.
- W2076230829 hasOpenAccess W2076230829 @default.
- W2076230829 hasPrimaryLocation W20762308291 @default.
- W2076230829 hasRelatedWork W2009889818 @default.
- W2076230829 hasRelatedWork W2076230829 @default.
- W2076230829 hasRelatedWork W2128924758 @default.
- W2076230829 hasRelatedWork W2350871275 @default.
- W2076230829 hasRelatedWork W2361137867 @default.
- W2076230829 hasRelatedWork W2367826004 @default.
- W2076230829 hasRelatedWork W2367934744 @default.
- W2076230829 hasRelatedWork W2368460920 @default.
- W2076230829 hasRelatedWork W2414007645 @default.
- W2076230829 hasRelatedWork W1929147813 @default.
- W2076230829 hasVolume "5" @default.
- W2076230829 isParatext "false" @default.
- W2076230829 isRetracted "false" @default.
- W2076230829 magId "2076230829" @default.
- W2076230829 workType "article" @default.