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- W2076236502 abstract "Abstract Using detergent removal by dialysis we have succeeded in making liposomes under very mild conditions, compatible with the introduction of macromolecules such as antibodies or RNA. We observed that in the presence of detergents, Bio-Beads SM-2® adsorb both octylglucoside and Triton X-100 as well as phospholipids. We used octylglucoside, which can be removed by dialysis more quickly than any other detergent, and can be completely eliminated, unlike the detergents ordinarily used, such as Triton X-100 and sodium deoxycholate. Bio-Beads allowed the complete removal of detergent when placed outside the dialysis bag. Under these conditions, dialysis resulted in the formation of large unilamellar liposomes. Their size depended mainly on the composition of the lipids. The largest liposomes corresponded to a lipid mixture of phosphatidylcholine, phosphatidylserine and cholesterol in a molar ratio of 1:1:1. Entrapment measurements and electron microscopic studies showed that these unilamellar liposomes were very large (1000 nm diameter) and that 45–50% of the macromolecular solutions could be entrapped with 13 mM phospholipids (35 l/mol lipid). These liposomes exhibited all the properties required for the delivery of macromolecules into cultured cells." @default.
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- W2076236502 date "1983-10-01" @default.
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- W2076236502 title "A very mild method allowing the encapsulation of very high amounts of macromolecules into very large (1000 nm) unilamellar liposomes" @default.
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- W2076236502 doi "https://doi.org/10.1016/0005-2736(83)90111-6" @default.
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