FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076241604> ?p ?o ?g. }

• W2076241604 endingPage "265" @default.
• W2076241604 startingPage "257" @default.
• W2076241604 abstract "Liquid chromatography/mass spectrometry (LC/MS) has been successfully applied to the detection of anabolic steroids in biological samples. However, the sensitive detection of saturated hydroxysteroids, such as androstanediols, by electrospray ionisation (ESI) is difficult because of their poor ability to ionise. In view of this, chemical derivatisation has been used to enhance the detection sensitivity of hydroxysteroids by LC/MS. This paper describes the development of a sensitive ultra-high-performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS) method for the screening of anabolic steroids in horse urine by incorporating a chemical derivatisation step, using picolinic acid as the derivatisation reagent. The method involved solid-phase extraction (SPE) of both free and conjugated anabolic steroids in horse urine using a polymer-based SPE cartridge (Abs Elut Nexus). The conjugated steroids in the eluate were hydrolysed by methanolysis and the resulting extract was further cleaned up by liquid-liquid extraction. The resulting free steroids in the extract were derivatised with picolinic acid to form the corresponding picolinoyl esters and analysed by UHPLC/MS/MS in the positive ESI mode with selected-reaction-monitoring. Separation of the targeted steroids was performed on a C18 UHPLC column. The instrument turnaround time was 10.5 min inclusive of post-run equilibration. A total of thirty-three anabolic steroids (including 17β-estradiol, 5(10)-estrene-3β,17α-diol, 5α-estrane-3β,17α-diol, 17α-ethyl-5α-estran-3α,17β-diol, 17α-methyl-5α-androstan-3,17β-diols, androstanediols, nandrolone and testosterone) spiked in negative horse urine at the QC levels (ranging from 0.75 to 30 ng/mL) could be consistently detected. The intra-day and inter-day precisions (% RSD) for the peak area ratios were around 7–51% and around 1–72%, respectively. The intra-day and inter-day precisions (% RSD) for the relative retention times were both less than 1% for all analytes, except the inter-day precision for boldione at 1.2%. The extraction recoveries for all targets were not less than 48%. With exceptional separation achieved by the UHPLC system, matrix interferences were minimal at the expected retention times of the selected transitions. As detection was performed with an UHPLC system coupled to a fast-scanning triple quadrupole mass spectrometer, the method could easily be expanded to accommodate additional steroid targets. This method has been validated for recovery and precision, and could be used regularly for doping control testing of anabolic steroids in horse urine samples." @default.
• W2076241604 created "2016-06-24" @default.
• W2076241604 creator A5041039815 @default.
• W2076241604 creator A5057417459 @default.
• W2076241604 creator A5061136707 @default.
• W2076241604 creator A5066179729 @default.
• W2076241604 creator A5070948290 @default.
• W2076241604 creator A5086680106 @default.
• W2076241604 date "2012-04-01" @default.
• W2076241604 modified "2023-10-01" @default.
• W2076241604 title "Rapid screening of anabolic steroids in horse urine with ultra-high-performance liquid chromatography/tandem mass spectrometry after chemical derivatisation" @default.
• W2076241604 cites W1965221481 @default.
• W2076241604 cites W1968700231 @default.
• W2076241604 cites W1976803011 @default.
• W2076241604 cites W1978203908 @default.
• W2076241604 cites W1983213434 @default.
• W2076241604 cites W1998714100 @default.
• W2076241604 cites W2018266297 @default.
• W2076241604 cites W2020742672 @default.
• W2076241604 cites W2031500928 @default.
• W2076241604 cites W2034218853 @default.
• W2076241604 cites W2051985525 @default.
• W2076241604 cites W2052852603 @default.
• W2076241604 cites W2054599893 @default.
• W2076241604 cites W2059822016 @default.
• W2076241604 cites W2061400317 @default.
• W2076241604 cites W2070584004 @default.
• W2076241604 cites W2072278348 @default.
• W2076241604 cites W2081784418 @default.
• W2076241604 cites W2085393713 @default.
• W2076241604 cites W2091870268 @default.
• W2076241604 cites W2128816357 @default.
• W2076241604 cites W2143732304 @default.
• W2076241604 cites W2151793021 @default.
• W2076241604 cites W2161165133 @default.
• W2076241604 cites W2951035198 @default.
• W2076241604 doi "https://doi.org/10.1016/j.chroma.2011.12.095" @default.
• W2076241604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22265177" @default.
• W2076241604 hasPublicationYear "2012" @default.
• W2076241604 type Work @default.
• W2076241604 sameAs 2076241604 @default.
• W2076241604 citedByCount "35" @default.
• W2076241604 countsByYear W20762416042013 @default.
• W2076241604 countsByYear W20762416042015 @default.
• W2076241604 countsByYear W20762416042016 @default.
• W2076241604 countsByYear W20762416042017 @default.
• W2076241604 countsByYear W20762416042018 @default.
• W2076241604 countsByYear W20762416042019 @default.
• W2076241604 countsByYear W20762416042020 @default.
• W2076241604 countsByYear W20762416042021 @default.
• W2076241604 countsByYear W20762416042022 @default.
• W2076241604 countsByYear W20762416042023 @default.
• W2076241604 crossrefType "journal-article" @default.
• W2076241604 hasAuthorship W2076241604A5041039815 @default.
• W2076241604 hasAuthorship W2076241604A5057417459 @default.
• W2076241604 hasAuthorship W2076241604A5061136707 @default.
• W2076241604 hasAuthorship W2076241604A5066179729 @default.
• W2076241604 hasAuthorship W2076241604A5070948290 @default.
• W2076241604 hasAuthorship W2076241604A5086680106 @default.
• W2076241604 hasConcept C1008401 @default.
• W2076241604 hasConcept C162356407 @default.
• W2076241604 hasConcept C185592680 @default.
• W2076241604 hasConcept C2779480814 @default.
• W2076241604 hasConcept C2780026642 @default.
• W2076241604 hasConcept C31827203 @default.
• W2076241604 hasConcept C43617362 @default.
• W2076241604 hasConcept C55493867 @default.
• W2076241604 hasConcept C64489805 @default.
• W2076241604 hasConcept C81426234 @default.
• W2076241604 hasConceptScore W2076241604C1008401 @default.
• W2076241604 hasConceptScore W2076241604C162356407 @default.
• W2076241604 hasConceptScore W2076241604C185592680 @default.
• W2076241604 hasConceptScore W2076241604C2779480814 @default.
• W2076241604 hasConceptScore W2076241604C2780026642 @default.
• W2076241604 hasConceptScore W2076241604C31827203 @default.
• W2076241604 hasConceptScore W2076241604C43617362 @default.
• W2076241604 hasConceptScore W2076241604C55493867 @default.
• W2076241604 hasConceptScore W2076241604C64489805 @default.
• W2076241604 hasConceptScore W2076241604C81426234 @default.
• W2076241604 hasLocation W20762416041 @default.
• W2076241604 hasLocation W20762416042 @default.
• W2076241604 hasOpenAccess W2076241604 @default.
• W2076241604 hasPrimaryLocation W20762416041 @default.
• W2076241604 hasRelatedWork W1964413082 @default.
• W2076241604 hasRelatedWork W1985716793 @default.
• W2076241604 hasRelatedWork W2008161654 @default.
• W2076241604 hasRelatedWork W2028520241 @default.
• W2076241604 hasRelatedWork W2132567408 @default.
• W2076241604 hasRelatedWork W2357498526 @default.
• W2076241604 hasRelatedWork W2582517916 @default.
• W2076241604 hasRelatedWork W2787820172 @default.
• W2076241604 hasRelatedWork W3092019062 @default.
• W2076241604 hasRelatedWork W3188441070 @default.
• W2076241604 hasVolume "1232" @default.
• W2076241604 isParatext "false" @default.
• W2076241604 isRetracted "false" @default.
• W2076241604 magId "2076241604" @default.
• W2076241604 workType "article" @default.