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- W2076245008 abstract "Protein A-tagging has become an important tool in characterization of protein–protein interactions in many systems, allowing purification of multicomponent complexes under native conditions. Here we provide a set of vectors that allow protein A-tagging in Candida albicans, through addition of the tag to open reading frames. These vectors were successfully used to generate stably tagged proteins that were functional, shown to be localized appropriately or assembled into complexes. These new vectors comprise a useful addition to the C. albicans molecular toolbox. Copyright © 2003 John Wiley & Sons, Ltd." @default.
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- W2076245008 date "2003-01-01" @default.
- W2076245008 modified "2023-10-11" @default.
- W2076245008 title "Protein A-tagging for purification of native macromolecular complexes fromCandida albicans" @default.
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- W2076245008 doi "https://doi.org/10.1002/yea.1036" @default.
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