FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076253303> ?p ?o ?g. }

• W2076253303 endingPage "334" @default.
• W2076253303 startingPage "325" @default.
• W2076253303 abstract "The effects of the diatomic radical, nitric oxide (NO), on melphalan-induced cytotoxicity in Chinese hamster V79 and human MCF-7 breast cancer cells were studied using clonogenic assays. NO delivered by the NO-releasing agent (C2H5)2N[N(O)NO]- Na+ (DEA/NO; 1 mM) resulted in enhancement of melphalan-mediated toxicity in Chinese hamster V79 lung fibroblasts and human breast cancer (MCF-7) cells by 3.6- and 4.3-fold, respectively, at the IC50 level. Nitrite/nitrate and diethylamine, the ultimate end products of DEA/NO decomposition, had little effect on melphalan cytotoxicity, which suggests that NO was responsible for the sensitization. Whereas maximal sensitization of melphalan cytotoxicity by DEA/NO was observed for simultaneous exposure of DEA/NO and melphalan, cells pretreated with DEA/NO were sensitized to melphalan for several hours after NO exposure. Reversing the order of treatment also resulted in a time-dependent enhancement in melphalan cytotoxicity. To explore possible mechanisms of NO enhancement of melphalan cytotoxicity, the effects of DEA/NO on three factors that might influence melphalan toxicity were examined, namely NO-mediated cell cycle perturbations, intracellular glutathione (GSH) levels and melphalan uptake. NO pretreatment resulted in a delayed entry into S phase and a G2/M block for both V79 and MCF-7 cells; however, cell cycle redistribution for V79 cells occurred after the cells returned to a level of cell survival, consistent with treatment with melphalan alone. After 15 min exposure of V79 cells to DEA/NO (1 mM), GSH levels were reduced to 40% of control values; however, GSH levels recovered fully after 1 h and were elevated 2 h after DEA/NO incubation. In contrast, DEA/NO (1 mM) incubation did not reduce GSH levels significantly in MCF-7 cells (approximately 10%). Melphalan uptake was increased by 33% after DEA/NO exposure in V79 cells. From these results enhancement of melphalan cytotoxicity mediated by NO appears to be complex and may involve several pathways, including possibly alteration of the repair of melphalan-induced lesions. Our observations may give insights for improving tumour kill with melphalan using either exogenous or possibly endogenous sources of NO." @default.
• W2076253303 created "2016-06-24" @default.
• W2076253303 creator A5000621452 @default.
• W2076253303 creator A5014699467 @default.
• W2076253303 creator A5039016487 @default.
• W2076253303 creator A5044929543 @default.
• W2076253303 creator A5046742103 @default.
• W2076253303 creator A5072939048 @default.
• W2076253303 creator A5073840390 @default.
• W2076253303 creator A5081250782 @default.
• W2076253303 date "1997-08-01" @default.
• W2076253303 modified "2023-10-10" @default.
• W2076253303 title "Nitric oxide enhancement of melphalan-induced cytotoxicity" @default.
• W2076253303 cites W1488610309 @default.
• W2076253303 cites W150673205 @default.
• W2076253303 cites W1545012941 @default.
• W2076253303 cites W1594268419 @default.
• W2076253303 cites W1607307372 @default.
• W2076253303 cites W1953775887 @default.
• W2076253303 cites W1973812004 @default.
• W2076253303 cites W1979780797 @default.
• W2076253303 cites W1994303699 @default.
• W2076253303 cites W1997266239 @default.
• W2076253303 cites W1998879311 @default.
• W2076253303 cites W2011233979 @default.
• W2076253303 cites W2033381628 @default.
• W2076253303 cites W2045001104 @default.
• W2076253303 cites W2047466663 @default.
• W2076253303 cites W2060334455 @default.
• W2076253303 cites W2062381728 @default.
• W2076253303 cites W2064871821 @default.
• W2076253303 cites W2075244890 @default.
• W2076253303 cites W2078298214 @default.
• W2076253303 cites W2079595986 @default.
• W2076253303 cites W2088975387 @default.
• W2076253303 cites W2091166505 @default.
• W2076253303 cites W2096202298 @default.
• W2076253303 cites W2099048452 @default.
• W2076253303 cites W2113796216 @default.
• W2076253303 cites W2133257374 @default.
• W2076253303 cites W2147015711 @default.
• W2076253303 cites W2162120925 @default.
• W2076253303 cites W2167120728 @default.
• W2076253303 doi "https://doi.org/10.1038/bjc.1997.386" @default.
• W2076253303 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2224050" @default.
• W2076253303 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9252199" @default.
• W2076253303 hasPublicationYear "1997" @default.
• W2076253303 type Work @default.
• W2076253303 sameAs 2076253303 @default.
• W2076253303 citedByCount "45" @default.
• W2076253303 countsByYear W20762533032012 @default.
• W2076253303 countsByYear W20762533032013 @default.
• W2076253303 countsByYear W20762533032014 @default.
• W2076253303 countsByYear W20762533032015 @default.
• W2076253303 countsByYear W20762533032016 @default.
• W2076253303 countsByYear W20762533032017 @default.
• W2076253303 countsByYear W20762533032018 @default.
• W2076253303 countsByYear W20762533032020 @default.
• W2076253303 crossrefType "journal-article" @default.
• W2076253303 hasAuthorship W2076253303A5000621452 @default.
• W2076253303 hasAuthorship W2076253303A5014699467 @default.
• W2076253303 hasAuthorship W2076253303A5039016487 @default.
• W2076253303 hasAuthorship W2076253303A5044929543 @default.
• W2076253303 hasAuthorship W2076253303A5046742103 @default.
• W2076253303 hasAuthorship W2076253303A5072939048 @default.
• W2076253303 hasAuthorship W2076253303A5073840390 @default.
• W2076253303 hasAuthorship W2076253303A5081250782 @default.
• W2076253303 hasBestOaLocation W20762533032 @default.
• W2076253303 hasConcept C109316439 @default.
• W2076253303 hasConcept C126322002 @default.
• W2076253303 hasConcept C153911025 @default.
• W2076253303 hasConcept C178790620 @default.
• W2076253303 hasConcept C181199279 @default.
• W2076253303 hasConcept C185592680 @default.
• W2076253303 hasConcept C202751555 @default.
• W2076253303 hasConcept C203014093 @default.
• W2076253303 hasConcept C2776364478 @default.
• W2076253303 hasConcept C2776694085 @default.
• W2076253303 hasConcept C2776755627 @default.
• W2076253303 hasConcept C2777755551 @default.
• W2076253303 hasConcept C2778575167 @default.
• W2076253303 hasConcept C2778670039 @default.
• W2076253303 hasConcept C2778684742 @default.
• W2076253303 hasConcept C29730261 @default.
• W2076253303 hasConcept C538909803 @default.
• W2076253303 hasConcept C55493867 @default.
• W2076253303 hasConcept C71924100 @default.
• W2076253303 hasConcept C86803240 @default.
• W2076253303 hasConcept C98274493 @default.
• W2076253303 hasConceptScore W2076253303C109316439 @default.
• W2076253303 hasConceptScore W2076253303C126322002 @default.
• W2076253303 hasConceptScore W2076253303C153911025 @default.
• W2076253303 hasConceptScore W2076253303C178790620 @default.
• W2076253303 hasConceptScore W2076253303C181199279 @default.
• W2076253303 hasConceptScore W2076253303C185592680 @default.
• W2076253303 hasConceptScore W2076253303C202751555 @default.
• W2076253303 hasConceptScore W2076253303C203014093 @default.
• W2076253303 hasConceptScore W2076253303C2776364478 @default.

• next