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- W2076259660 abstract "Five triterpenoids; Congmuyanoside V, X, XI, XII, and XV (Cong. V, X, XI, XII, and XV) were isolated from the root bark of Aralia elata. The effect of these triterpenoids on stimulus-induced superoxide generation in human neutrophils was assayed by measuring the reduction of ferricytochrome c (cyt. c) using a dual-beam spectrophotometer. Translocation of p47(phox), p67(phox), and rac to the cell membrane and tyrosyl or serine/threonine phosphorylation of neutrophil proteins were investigated using specific monoclonal antibodies. The five triterpenoids used in the present experiment significantly suppressed N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced superoxide generation in concentration-dependent manner. Congmuyanoside V, XII, and XV also suppressed arachidonic acid (AA)-induced superoxide generation in high concentration. However, these triterpenoids showed no effect on phorbol 12-myristate 13-acetate (PMA)-induced superoxide generation. fMLP- and AA-induced tyrosyl phosphorylation and translocation of the cytosolic proteins p47(phox), p67(phox), and rac to the cell membrane were suppressed in parallel with the suppression of the stimulus-induced superoxide generation." @default.
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- W2076259660 date "2006-02-01" @default.
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- W2076259660 title "Effect of five triterpenoid compounds isolated from root bark of Aralia elata on stimulus-induced superoxide generation, tyrosyl or serine/threonine phosphorylation and translocation of p47phox, p67phox, and rac to cell membrane in human neutrophils" @default.
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- W2076259660 doi "https://doi.org/10.1016/j.abb.2005.11.006" @default.
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