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• W2076277268 abstract "Vesicle shedding from bacteria is a universal process in most Gram-negative bacteria and a few Gram-positive bacteria. In this report, we isolate extracellular membrane vesicles (MVs) from the supernatants of Gram-positive pathogen Clostridium perfringens (C. perfringens). We demonstrated vesicle production in a variety of virulent and nonvirulent type A strains. MVs did not contain alpha-toxin and NetB toxin demonstrated by negative reaction to specific antibody and absence of specific proteins identified by LC-MS/MS. C. perfringens MVs contained DNA components such as 16S ribosomal RNA gene (16S rRNA), alpha-toxin gene (plc) and the perfringolysin O gene (pfoA) demonstrated by PCR. We also identified a total of 431 proteins in vesicles by 1-D gel separation and LC-MS/MS analysis. In vitro studies demonstrated that vesicles could be internalized into murine macrophage RAW264.7 cells without direct cytotoxicity effects, causing release of inflammation cytokines including granulocyte colony stimulating factor (G-CSF), tumor necrosis factor-alpha (TNF-α) and interleukin-1 (IL-1), which could also be detected in mice injected with MVs through intraperitoneal (i.p.) route. Mice immunized with C. perfringens MVs produced high titer IgG, especially IgG1, antibodies against C. perfringens membrane proteins. However, this kind of antibody could not provide protection in mice following challenge, though it could slightly postpone the time of death. Our results indicate that release of MVs from C. perfringens could provide a previously unknown mechanism to induce release of inflammatory cytokines, especially TNF-α, these findings may contribute to a better understanding of the pathogenesis of C. perfringens infection." @default.
• W2076277268 created "2016-06-24" @default.
• W2076277268 creator A5035189002 @default.
• W2076277268 creator A5038877679 @default.
• W2076277268 creator A5039529339 @default.
• W2076277268 creator A5044344943 @default.
• W2076277268 date "2014-05-01" @default.
• W2076277268 modified "2023-10-09" @default.
• W2076277268 title "Membrane vesicles of Clostridium perfringens type A strains induce innate and adaptive immunity" @default.
• W2076277268 cites W1486649516 @default.
• W2076277268 cites W1567134389 @default.
• W2076277268 cites W1860874235 @default.
• W2076277268 cites W1967006152 @default.
• W2076277268 cites W1969492177 @default.
• W2076277268 cites W1974161967 @default.
• W2076277268 cites W1984561439 @default.
• W2076277268 cites W1987028223 @default.
• W2076277268 cites W1988192415 @default.
• W2076277268 cites W1997969528 @default.
• W2076277268 cites W1999225385 @default.
• W2076277268 cites W2000332508 @default.
• W2076277268 cites W2008989704 @default.
• W2076277268 cites W2012176032 @default.
• W2076277268 cites W2014100067 @default.
• W2076277268 cites W2015313456 @default.
• W2076277268 cites W2022050193 @default.
• W2076277268 cites W2029626396 @default.
• W2076277268 cites W2030865291 @default.
• W2076277268 cites W2034539700 @default.
• W2076277268 cites W2035879375 @default.
• W2076277268 cites W2039789500 @default.
• W2076277268 cites W2044365036 @default.
• W2076277268 cites W2049416809 @default.
• W2076277268 cites W2051689433 @default.
• W2076277268 cites W2051710983 @default.
• W2076277268 cites W2054836349 @default.
• W2076277268 cites W2055923937 @default.
• W2076277268 cites W2056123535 @default.
• W2076277268 cites W2059210701 @default.
• W2076277268 cites W2062886408 @default.
• W2076277268 cites W2063490043 @default.
• W2076277268 cites W2073273682 @default.
• W2076277268 cites W2074182306 @default.
• W2076277268 cites W2074885818 @default.
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• W2076277268 cites W2080885706 @default.
• W2076277268 cites W2081157135 @default.
• W2076277268 cites W2083657436 @default.
• W2076277268 cites W2085060526 @default.
• W2076277268 cites W2087560843 @default.
• W2076277268 cites W2089492568 @default.
• W2076277268 cites W2089522808 @default.
• W2076277268 cites W2093083597 @default.
• W2076277268 cites W2094650098 @default.
• W2076277268 cites W2096749374 @default.
• W2076277268 cites W2097197658 @default.
• W2076277268 cites W2098571492 @default.
• W2076277268 cites W2100706861 @default.
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• W2076277268 cites W2112078820 @default.
• W2076277268 cites W2115765556 @default.
• W2076277268 cites W2118636688 @default.
• W2076277268 cites W2127771209 @default.
• W2076277268 cites W2129027970 @default.
• W2076277268 cites W2130723556 @default.
• W2076277268 cites W2133392388 @default.
• W2076277268 cites W2136120771 @default.
• W2076277268 cites W2136723907 @default.
• W2076277268 cites W2137182736 @default.
• W2076277268 cites W2139231554 @default.
• W2076277268 cites W2141998772 @default.
• W2076277268 cites W2143311335 @default.
• W2076277268 cites W2145536472 @default.
• W2076277268 cites W2149167841 @default.
• W2076277268 cites W2149778210 @default.
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• W2076277268 cites W2151817333 @default.
• W2076277268 cites W2152267193 @default.
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• W2076277268 cites W2163321950 @default.
• W2076277268 cites W2167335500 @default.
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• W2076277268 doi "https://doi.org/10.1016/j.ijmm.2014.02.006" @default.
• W2076277268 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4285460" @default.
• W2076277268 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24631214" @default.
• W2076277268 hasPublicationYear "2014" @default.
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