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- W2076291680 abstract "Abstract The degree of edema occurring in dog kidneys after perfusion with cryoprotector-containing electrolyte medium is illustrated by the reduction in cellular protein, DNA, and RNA concentration. Perfusion alone only slightly affects the metabolic activities. After slow cooling and preservation at −196 °C, the amino acid incorporation into the proteins of cortex and to a lesser degree of medulla, is considerably inhibited. In identical conditions the membrane transport functions seem to be more successfully protected. The thymidine incorporation into DNA can even be stimulated, but this may be a detrimental irritatory factor since it is accompanied by reduced protein synthesizing activity. By accelerating the cooling rate, cryoinjury increased alarmingly without affecting the incorporation of thymidine into DNA. It is concluded that even if vascular problems have prevented the successful cryopreservation of complex mammalian structures up to now, the degree to which metabolic activities can be protected is promising for further development." @default.
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- W2076291680 date "1980-12-01" @default.
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- W2076291680 title "Metabolic changes in dog kidney during cryopreservation" @default.
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- W2076291680 doi "https://doi.org/10.1016/0011-2240(80)90069-3" @default.
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