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• W2076298024 endingPage "201" @default.
• W2076298024 startingPage "189" @default.
• W2076298024 abstract "Seven groups, each consisting of two to nine unrelated HLA-A, -B, and -DR serologically identical individuals, were analyzed by DNA-restriction fragment length polymorphism (RFLP) and mixed lymphocyte reactions (MLR) in order to evaluate HLA-class II identity between unrelated individuals and to asses the importance of HLA-class II incompatibilities detected by DNA-RFLP in the allogeneic reactions. It is clear that DNA-RFLP represents a powerful typing method for HLA-DR, -DQ, and -DP ssince the combinations of the RFLP band patterns define all the serological specificities and most of the cellular specificities togive a highly accurate typing. This report shows that an HLA-DP incompatibility induces proliferation in primary mixed lymphocyte culture (MLC) between unrelated HLA-A, -B, -DR, -DQ, and -DW identical individuals, which may suggest the imortance of this molecule as a transplantation antigen, especially for unrelated bone m̀arrow transplantations. Still, an isolated HLA-DPw4/HLA-DPa disparity did not induce any proliferation in MLC. Moreover, our results show that DQw7 (w3)/DQw8 (w3) disparity associated with HLA-DR4 represents a nonfunctional incompatibility in MLR. The HLA-Dw subtypes of HLA-DR specificities can induce a high proliferative response in MLC. Finally, DNA-RFLP typing represents a reliable method for the selection of histocompatible donor-recipient pairs and could potentially reduce many logistic problems and delays in live-donor transplantation, especially for unrelated bone marrow transplantation." @default.
• W2076298024 created "2016-06-24" @default.
• W2076298024 creator A5017519158 @default.
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• W2076298024 date "1990-11-01" @default.
• W2076298024 modified "2023-09-27" @default.
• W2076298024 title "Evaluation of HLA-class II identity between unrelated individuals by serological typing, DNA-RFLP method, and mixed lymphocyte reaction" @default.
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• W2076298024 doi "https://doi.org/10.1016/0198-8859(90)90114-5" @default.
• W2076298024 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1980919" @default.
• W2076298024 hasPublicationYear "1990" @default.
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