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• W2076317997 abstract "Conidial germination, appressorial formation, and secondary sporulation in Zoophthora radicans occurred independently of DNA replication and nuclear division. Video-image analysis combined with epifluorescence microcopy showed that at 18 hr postinoculation, approximately 10% of germlings had undergone the first DNA duplication and nuclear division and that 20% of germlings had formed appressoria. Hydroxyurea, an inhibitor of DNA synthesis, prevented DNA replication and nuclear division but allowed production of germ tubes and appressoria. Actinomycin D, an inhibitor of RNA synthesis, totally inhibited germination at concentrations above 10 μg/ml. At lower concentrations, actinomycin D allowed formation of germ tube and appressoria in a dose-dependent fashion. However, even at the lowest concentration tested (1 μg/ml), nuclear division and formation of penetration hyphae were prevented. Formation of secondary conidia and capilliconidia was also independent of nuclear division but was totally inhibited by drugs blocking RNA or protein synthesis. The implications of differentiation without prior nuclear division are discussed." @default.
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• W2076317997 date "1991-01-01" @default.
• W2076317997 modified "2023-09-23" @default.
• W2076317997 title "Nuclear events during germination and appressorial formation of the entomopathogenic fungus Zoophthora radicans (Zygomycetes: Entomophthorales)" @default.
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• W2076317997 doi "https://doi.org/10.1016/0022-2011(91)90039-s" @default.
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