Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076353048> ?p ?o ?g. }
Showing items 1 to 70 of
70
with 100 items per page.
- W2076353048 endingPage "566" @default.
- W2076353048 startingPage "561" @default.
- W2076353048 abstract "Fixation by periodate/lysine/paraformaldehyde, a method purported to cross-link specifically plasma membrane glycoproteins, was evaluated using Novikoff rat ascites hepatocellular carcinoma cells. Cells were treated with periodate/lysine, periodate/glycine, and periodate/lysine/paraformaldehyde and subsequently reduced with NaB3H4. The glycoproteins labeled with 3H were resolved by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and visualized by fluorography. The effects of reactant concentrations on 3H-labeling of cellular components, cell viability, and cross-linkage of 3H-labeled proteins were examined. The effect of increasing the localized density of plasma membrane glycoproteins on the extent of cross-linkage by periodate and lysine was investigated using cells in which patching of the plasma membrane glycoproteins had been induced by ferritin-conjugated concanavalin A/rabbit antiferritin antiserum. Also investigated was the periodate-independent to mixtures of periodate and lysine or glycine. Results of these studies did not support a mechanism of cross-linking involving reaction between the free base lysin and aldehyde groups on periodate oxidized carbohydrate residues but suggested a complex interaction between periodate oxidized plasma membrane glycoproteins and polymeric complexes of lysine and formaldehyde.U" @default.
- W2076353048 created "2016-06-24" @default.
- W2076353048 creator A5010257030 @default.
- W2076353048 creator A5043660511 @default.
- W2076353048 creator A5052531267 @default.
- W2076353048 creator A5056819496 @default.
- W2076353048 creator A5058686249 @default.
- W2076353048 date "1981-04-01" @default.
- W2076353048 modified "2023-09-24" @default.
- W2076353048 title "Evaluation of periodate/lysine/paraformaldehyde fixation as a method for cross-linking plasma membrane glycoproteins." @default.
- W2076353048 doi "https://doi.org/10.1177/29.4.6265547" @default.
- W2076353048 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6265547" @default.
- W2076353048 hasPublicationYear "1981" @default.
- W2076353048 type Work @default.
- W2076353048 sameAs 2076353048 @default.
- W2076353048 citedByCount "24" @default.
- W2076353048 countsByYear W20763530482012 @default.
- W2076353048 countsByYear W20763530482018 @default.
- W2076353048 countsByYear W20763530482020 @default.
- W2076353048 crossrefType "journal-article" @default.
- W2076353048 hasAuthorship W2076353048A5010257030 @default.
- W2076353048 hasAuthorship W2076353048A5043660511 @default.
- W2076353048 hasAuthorship W2076353048A5052531267 @default.
- W2076353048 hasAuthorship W2076353048A5056819496 @default.
- W2076353048 hasAuthorship W2076353048A5058686249 @default.
- W2076353048 hasBestOaLocation W20763530481 @default.
- W2076353048 hasConcept C108625454 @default.
- W2076353048 hasConcept C116084860 @default.
- W2076353048 hasConcept C178790620 @default.
- W2076353048 hasConcept C185592680 @default.
- W2076353048 hasConcept C2776016237 @default.
- W2076353048 hasConcept C2776661909 @default.
- W2076353048 hasConcept C2776678281 @default.
- W2076353048 hasConcept C2776789926 @default.
- W2076353048 hasConcept C43617362 @default.
- W2076353048 hasConcept C515207424 @default.
- W2076353048 hasConcept C55493867 @default.
- W2076353048 hasConceptScore W2076353048C108625454 @default.
- W2076353048 hasConceptScore W2076353048C116084860 @default.
- W2076353048 hasConceptScore W2076353048C178790620 @default.
- W2076353048 hasConceptScore W2076353048C185592680 @default.
- W2076353048 hasConceptScore W2076353048C2776016237 @default.
- W2076353048 hasConceptScore W2076353048C2776661909 @default.
- W2076353048 hasConceptScore W2076353048C2776678281 @default.
- W2076353048 hasConceptScore W2076353048C2776789926 @default.
- W2076353048 hasConceptScore W2076353048C43617362 @default.
- W2076353048 hasConceptScore W2076353048C515207424 @default.
- W2076353048 hasConceptScore W2076353048C55493867 @default.
- W2076353048 hasIssue "4" @default.
- W2076353048 hasLocation W20763530481 @default.
- W2076353048 hasLocation W20763530482 @default.
- W2076353048 hasOpenAccess W2076353048 @default.
- W2076353048 hasPrimaryLocation W20763530481 @default.
- W2076353048 hasRelatedWork W1483633732 @default.
- W2076353048 hasRelatedWork W1536607027 @default.
- W2076353048 hasRelatedWork W1561056382 @default.
- W2076353048 hasRelatedWork W1989429815 @default.
- W2076353048 hasRelatedWork W1990563422 @default.
- W2076353048 hasRelatedWork W2000677669 @default.
- W2076353048 hasRelatedWork W2012250101 @default.
- W2076353048 hasRelatedWork W2056828959 @default.
- W2076353048 hasRelatedWork W2158081554 @default.
- W2076353048 hasRelatedWork W2952588630 @default.
- W2076353048 hasVolume "29" @default.
- W2076353048 isParatext "false" @default.
- W2076353048 isRetracted "false" @default.
- W2076353048 magId "2076353048" @default.
- W2076353048 workType "article" @default.