FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076356476> ?p ?o ?g. }

• W2076356476 endingPage "1002" @default.
• W2076356476 startingPage "996" @default.
• W2076356476 abstract "The structural features of the active site of human monoamine oxidase B (MAO-B) were investigated by affinity labeling and site-directed mutagenesis. The pseudosubstrate inhibitor N-[2-aminoethyl]-5-chloro-2-pyridine carboxamide HCl (lazabemide) can be irreversibly linked to MAO-B by reduction of the enzyme-inhibitor complex with NaBH(3)CN. Analysis of the flavin spectrum of [(3)H]lazabemide-labeled human MAO-B indicated that insertion of the inhibitor did not occur into the isoalloxazine ring of FAD. After trypsin digestion and HPLC peptide mapping of the radiolabeled enzyme, two labeled peptides were observed. Sequence analysis showed that both peptides started at Val371 of human MAO-B. These results indicate that [(3)H]lazabemide is incorporated into the MAO-B peptide stretch containing the FAD-modified Cys397. The function of putative active-site residues contained in this region was investigated by site-directed mutagenesis and expression of the mutant proteins in HEK-293 cells. Substitution of His382 of MAO-B with an Arg greatly reduced the enzymic activity, suggesting that this residue may represent a nucleophile relevant for the MAO-B catalytic mechanism. Whereas it has been shown that mutation of Cys389 with a Ser residue does not markedly affect the activity of the enzyme [Wu, H.-F., Chen, K. and Shih, J.C. (1993) Mol. Pharmacol. 43, 888-893] the mutant carrying an Ala at this position was virtually inactive. Conversely, substitution of Lys386 (to Met) and Ser394 (to Ala) did not markedly modify the kinetic properties of the enzyme. We also report that mutation of MAO-B Thr158 (to Ala) resulted in a dramatic loss of enzymic activity." @default.
• W2076356476 created "2016-06-24" @default.
• W2076356476 creator A5003329444 @default.
• W2076356476 creator A5004843541 @default.
• W2076356476 creator A5017780434 @default.
• W2076356476 creator A5033310705 @default.
• W2076356476 creator A5034204219 @default.
• W2076356476 creator A5070164555 @default.
• W2076356476 creator A5084424541 @default.
• W2076356476 creator A5089803319 @default.
• W2076356476 date "1996-03-01" @default.
• W2076356476 modified "2023-10-03" @default.
• W2076356476 title "Investigation on the Structure of the Active Site of Monoamine Oxidase-B by Affinity Labeling with the Selective Inhibitor Lazabemide and by Site-Directed Mutagenesis" @default.
• W2076356476 cites W1550877969 @default.
• W2076356476 cites W1577728072 @default.
• W2076356476 cites W159198934 @default.
• W2076356476 cites W1915733116 @default.
• W2076356476 cites W1970718019 @default.
• W2076356476 cites W1975085160 @default.
• W2076356476 cites W1977190562 @default.
• W2076356476 cites W1981626891 @default.
• W2076356476 cites W1981765089 @default.
• W2076356476 cites W1987684118 @default.
• W2076356476 cites W2001727865 @default.
• W2076356476 cites W2003179603 @default.
• W2076356476 cites W2006392012 @default.
• W2076356476 cites W2008000048 @default.
• W2076356476 cites W2017028455 @default.
• W2076356476 cites W2020425958 @default.
• W2076356476 cites W2022491812 @default.
• W2076356476 cites W2024298846 @default.
• W2076356476 cites W2037039276 @default.
• W2076356476 cites W2042695207 @default.
• W2076356476 cites W2062852247 @default.
• W2076356476 cites W2070724870 @default.
• W2076356476 cites W2077260122 @default.
• W2076356476 cites W2088115099 @default.
• W2076356476 cites W2110899059 @default.
• W2076356476 cites W2128140057 @default.
• W2076356476 cites W2134996660 @default.
• W2076356476 cites W2142456895 @default.
• W2076356476 cites W2145854658 @default.
• W2076356476 cites W2164795037 @default.
• W2076356476 cites W2170014008 @default.
• W2076356476 cites W2326554068 @default.
• W2076356476 cites W2401973332 @default.
• W2076356476 cites W2951934822 @default.
• W2076356476 doi "https://doi.org/10.1111/j.1432-1033.1996.00996.x" @default.
• W2076356476 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8665924" @default.
• W2076356476 hasPublicationYear "1996" @default.
• W2076356476 type Work @default.
• W2076356476 sameAs 2076356476 @default.
• W2076356476 citedByCount "32" @default.
• W2076356476 countsByYear W20763564762017 @default.
• W2076356476 countsByYear W20763564762018 @default.
• W2076356476 countsByYear W20763564762020 @default.
• W2076356476 countsByYear W20763564762023 @default.
• W2076356476 crossrefType "journal-article" @default.
• W2076356476 hasAuthorship W2076356476A5003329444 @default.
• W2076356476 hasAuthorship W2076356476A5004843541 @default.
• W2076356476 hasAuthorship W2076356476A5017780434 @default.
• W2076356476 hasAuthorship W2076356476A5033310705 @default.
• W2076356476 hasAuthorship W2076356476A5034204219 @default.
• W2076356476 hasAuthorship W2076356476A5070164555 @default.
• W2076356476 hasAuthorship W2076356476A5084424541 @default.
• W2076356476 hasAuthorship W2076356476A5089803319 @default.
• W2076356476 hasBestOaLocation W20763564761 @default.
• W2076356476 hasConcept C103408237 @default.
• W2076356476 hasConcept C104317684 @default.
• W2076356476 hasConcept C107824862 @default.
• W2076356476 hasConcept C143065580 @default.
• W2076356476 hasConcept C16318435 @default.
• W2076356476 hasConcept C181199279 @default.
• W2076356476 hasConcept C185592680 @default.
• W2076356476 hasConcept C2776024655 @default.
• W2076356476 hasConcept C33093398 @default.
• W2076356476 hasConcept C39398028 @default.
• W2076356476 hasConcept C41183919 @default.
• W2076356476 hasConcept C55493867 @default.
• W2076356476 hasConcept C71240020 @default.
• W2076356476 hasConceptScore W2076356476C103408237 @default.
• W2076356476 hasConceptScore W2076356476C104317684 @default.
• W2076356476 hasConceptScore W2076356476C107824862 @default.
• W2076356476 hasConceptScore W2076356476C143065580 @default.
• W2076356476 hasConceptScore W2076356476C16318435 @default.
• W2076356476 hasConceptScore W2076356476C181199279 @default.
• W2076356476 hasConceptScore W2076356476C185592680 @default.
• W2076356476 hasConceptScore W2076356476C2776024655 @default.
• W2076356476 hasConceptScore W2076356476C33093398 @default.
• W2076356476 hasConceptScore W2076356476C39398028 @default.
• W2076356476 hasConceptScore W2076356476C41183919 @default.
• W2076356476 hasConceptScore W2076356476C55493867 @default.
• W2076356476 hasConceptScore W2076356476C71240020 @default.
• W2076356476 hasIssue "3" @default.
• W2076356476 hasLocation W20763564761 @default.
• W2076356476 hasLocation W20763564762 @default.
• W2076356476 hasOpenAccess W2076356476 @default.
• W2076356476 hasPrimaryLocation W20763564761 @default.

• next