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• W2076364053 abstract "Background: Recently, human herpesvirus 8 (HHV‐8) has been isolated from almost all cases of Kaposi’s sarcoma. It has not been found in most cutaneous hemangioproliferative disorders other than Kaposi’s sarcoma. Benign vascular lesions including Kimura’s disease were not found to contain the HHV‐8 DNA sequence. However, there has been contradictory data concerning the presence of HHV‐8 in angiolymphoid hyperplasia with eosinophilia (ALHE). Clonality studies in ALHE and Kimura’s disease were rare. Methods: We performed polymerase chain reaction (PCR)‐based analysis to determine whether HHV‐8 is present and heteroduplex analysis of rearranged T‐cell receptor (TCR) gene for clonality assessment in paraffin‐embedded skin biopsy samples of 7 ALHE and 2 Kimura’s disease, taken from immunocompetent patients. Results: HHV‐8 could not be identified in all the cases of ALHE and Kimura’s disease. Although 2 cases (2/7) of ALHE and 2 cases (2/2) of Kimura’s disease showed positive result for PCR analysis of TCR, all the cases were negative for heteroduplex‐PCR. Conclusions: We suggest that HHV‐8 may not involve in a pathogenetic role in ALHE and Kimura’s disease and the failure to demonstrate clonality may be consistent with the reactive nature of these diseases and lack of malignant transformation. In addition, heteroduplex‐PCR can be applied to confirm doubtful cases of lymphoma in that heteroduplex‐PCR is more specific than PCR as seen in our study." @default.
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• W2076364053 date "2001-08-01" @default.
• W2076364053 modified "2023-10-17" @default.
• W2076364053 title "Polymerase chain reaction (PCR) for human herpesvirus 8 and heteroduplex PCR for clonality assessment in angiolymphoid hyperplasia with eosinophilia and Kimura’s disease" @default.
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• W2076364053 doi "https://doi.org/10.1034/j.1600-0560.2001.280705.x" @default.
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