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- W2076372283 abstract "Glycolipid synthetases can be assayed conveniently by incubating the lipid substrate with the radiosugar-labeled nucleotide in a small plastic scintillation vial. At the end of the incubation period, water and perchloric acid are added, thenn-butanol, then a toluene-based scintillation cocktail. The radioactive lipid partitions into the scintillation fluid, leaving excess sugar nucleotide in the aqueous phase. Only a small fraction of the total radioactivity in the aqueous layer is detectable. This method is illustrated for ceramide: UDP-glucose glucosyltransferase. The approach should be applicable to other lipid synthetases that can be assayed with a radioactive hydrophilic substrate." @default.
- W2076372283 created "2016-06-24" @default.
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- W2076372283 date "1981-10-01" @default.
- W2076372283 modified "2023-09-26" @default.
- W2076372283 title "A 2-phase liquid scintillation assay for glycolipid synthetases" @default.
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- W2076372283 doi "https://doi.org/10.1007/bf02535347" @default.
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