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• W2076393726 abstract "The rate of the alkaline phosphatase-catalyzed hydrolysis of 4-methylumbelliferone phosphate was measured in acoustically levitated droplets of aqueous tris (50 mM) at pH 8.5 at 22 +/- 2 degrees C and in supercooled solution at -6 +/- 2 degrees C. At 22 degrees C, the rate of product formation was in excellent agreement with the rate observed in bulk solution in a cuvette, indicating that the acoustic levitation process does not alter the enzyme activity. The rate of the reaction decreased 6-fold in supercooled solution at -6 +/- 2 degrees C. The acoustic levitator apparatus is described in detail." @default.
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• W2076393726 date "2005-03-08" @default.
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• W2076393726 title "Enzyme Kinetics in Acoustically Levitated Droplets of Supercooled Water: A Novel Approach to Cryoenzymology" @default.
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• W2076393726 doi "https://doi.org/10.1021/ac048486f" @default.
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