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• W2076397981 abstract "The response of fifth larval instar locusts to injected adipokinetic hormone (AKH) is only poor, as is reflected in both a very moderate elevation of the haemolymph lipid concentration and the slight occurrence of the haemolymph lipophorin interconversions characteristic for adult locusts, resulting in formation of only small quantities of the low density lipophorin (A+). However, an additional lipophorin fraction (A′) is induced, which is intermediate in density and size between high and low density lipophorin and which is not identified in adult haemolymph. As in adults, larval A+ formation includes association of the resting high density lipophorin with a non-lipid containing protein (C2), the haemolymph concentration of which is only one-fifth relative to adults. However, the larval haemolymph protein composition is not the primary cause of the incomplete adipokinetic response, as elevation of the concentration of protein C2 by injection of isolated adult C2, whether or not in combination with adult high density lipophorin, did not increase lipophorin conversions nor haemolymph lipid elevation. In vitro incubation of larval fat bodies in adult haemolymph showed that competency to both the AKH-induced lipid release and the haemolymph lipophorin conversions of the larval fat body are reduced compared to equal amounts of adult tissue. Reciprocal incubation of adult fat body in larval haemolymph resulted in only a very moderate adipokinetic response, demonstrating that larval haemolymph protein composition is restrictive for full development of hormone action. Both immunoblotting experiments and enzyme-linked immunosorbent assays (ELISA), using monoclonal antibodies specific for the adult lipophorin apoproteins, indicated that the larval lipophorins closely resemble the adult forms. Apparently the structure of locust lipophorins is remarkably constant throughout development despite changes in metabolic functions." @default.
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• W2076397981 date "1987-01-01" @default.
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• W2076397981 title "Adipokinetic hormone-induced lipid mobilization and lipophorin interconversions in fifth larval instar locusts" @default.
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• W2076397981 doi "https://doi.org/10.1016/0020-1790(87)90014-x" @default.
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