FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076419068> ?p ?o ?g. }

• W2076419068 endingPage "952" @default.
• W2076419068 startingPage "936" @default.
• W2076419068 abstract "New conjugates of triplex-forming pyrimidine oligo(2′-O-methylribonucleotides) with one or two ‘head-to-head’ hairpin oligo(N-methylpyrrole carboxamide) minor-groove binders (MGBs) attached to the terminal phosphate of the oligonucleotides with a oligo(ethylene glycol) linker were synthesized. It was demonstrated that, under appropriate conditions, the conjugates form stable complexes with double-stranded DNA (dsDNA) similarly to triplex-forming oligo(deoxyribonucleotide) (TFO) conjugates containing 5-methylated cytosines. Kinetic and thermodynamic parameters of the complex formation were evaluated by gel-shift assay and thermal denaturation. Higher melting temperatures (Tm), faster complex formation, and lower dissociation constants (Kd) of the triple helices (6–7 nM) were observed for complexes of MGB-oligo(2′-O-methylribonucleotide) conjugates with the target dsDNA compared to the nonconjugated individual components. Interaction of MGB moieties with the HIV proviral DNA fragment was indicated by UV/VIS absorption changes at 320 nm in the melting curves. The introduction of thymidine via a 3′,3′-type ‘inverted’ phosphodiester linkage at the 3′-end of oligo(2′-O-methylribonucleotide) conjugates (3′-protection) had no strong influence on triplex formation, but slightly affected complex stability. At pH 6.0, when one or two hairpin MGBs were attached to the oligonucleotide, both triplex formation and minor-groove binding played important roles in complex formation. When two ‘head-to-head’ oligo(N-methylpyrrole) ligands were attached to the same terminal phosphate of the oligonucleotide or the linker, binding was observed at pH >7.5 and at high temperatures (up to 74°). However, under these conditions, binding was retained only by the MGB part of the conjugate." @default.
• W2076419068 created "2016-06-24" @default.
• W2076419068 creator A5004630053 @default.
• W2076419068 creator A5006154857 @default.
• W2076419068 creator A5008507130 @default.
• W2076419068 creator A5076452008 @default.
• W2076419068 creator A5081735234 @default.
• W2076419068 creator A5087640214 @default.
• W2076419068 creator A5090808491 @default.
• W2076419068 date "2005-07-01" @default.
• W2076419068 modified "2023-10-18" @default.
• W2076419068 title "Sequence-Specific Conjugates of Oligo(2′-O-methylribonucleotides) and Hairpin Oligocarboxamide Minor-Groove Binders: Design, Synthesis, and Binding Studies with Double-Stranded DNA" @default.
• W2076419068 cites W100720734 @default.
• W2076419068 cites W1973712778 @default.
• W2076419068 cites W1974507072 @default.
• W2076419068 cites W1975013620 @default.
• W2076419068 cites W1975642354 @default.
• W2076419068 cites W1978413122 @default.
• W2076419068 cites W1983595686 @default.
• W2076419068 cites W1985933273 @default.
• W2076419068 cites W1987139320 @default.
• W2076419068 cites W1987571328 @default.
• W2076419068 cites W1991312343 @default.
• W2076419068 cites W1994739183 @default.
• W2076419068 cites W1996007908 @default.
• W2076419068 cites W2002075021 @default.
• W2076419068 cites W2006668755 @default.
• W2076419068 cites W2011052879 @default.
• W2076419068 cites W2017426714 @default.
• W2076419068 cites W2038985545 @default.
• W2076419068 cites W2048738796 @default.
• W2076419068 cites W2049517953 @default.
• W2076419068 cites W2064375321 @default.
• W2076419068 cites W2070235053 @default.
• W2076419068 cites W2072791365 @default.
• W2076419068 cites W2077782229 @default.
• W2076419068 cites W2088076921 @default.
• W2076419068 cites W2093714254 @default.
• W2076419068 cites W2164577867 @default.
• W2076419068 cites W2165598929 @default.
• W2076419068 cites W2167796192 @default.
• W2076419068 cites W2176730350 @default.
• W2076419068 cites W2178778469 @default.
• W2076419068 cites W2195270156 @default.
• W2076419068 cites W2318987089 @default.
• W2076419068 cites W2402556329 @default.
• W2076419068 cites W1977743507 @default.
• W2076419068 doi "https://doi.org/10.1002/cbdv.200590071" @default.
• W2076419068 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17193185" @default.
• W2076419068 hasPublicationYear "2005" @default.
• W2076419068 type Work @default.
• W2076419068 sameAs 2076419068 @default.
• W2076419068 citedByCount "20" @default.
• W2076419068 countsByYear W20764190682012 @default.
• W2076419068 countsByYear W20764190682013 @default.
• W2076419068 countsByYear W20764190682014 @default.
• W2076419068 countsByYear W20764190682015 @default.
• W2076419068 countsByYear W20764190682016 @default.
• W2076419068 countsByYear W20764190682023 @default.
• W2076419068 crossrefType "journal-article" @default.
• W2076419068 hasAuthorship W2076419068A5004630053 @default.
• W2076419068 hasAuthorship W2076419068A5006154857 @default.
• W2076419068 hasAuthorship W2076419068A5008507130 @default.
• W2076419068 hasAuthorship W2076419068A5076452008 @default.
• W2076419068 hasAuthorship W2076419068A5081735234 @default.
• W2076419068 hasAuthorship W2076419068A5087640214 @default.
• W2076419068 hasAuthorship W2076419068A5090808491 @default.
• W2076419068 hasConcept C104317684 @default.
• W2076419068 hasConcept C111919701 @default.
• W2076419068 hasConcept C111921641 @default.
• W2076419068 hasConcept C129312508 @default.
• W2076419068 hasConcept C185592680 @default.
• W2076419068 hasConcept C21463570 @default.
• W2076419068 hasConcept C2779321679 @default.
• W2076419068 hasConcept C2780349520 @default.
• W2076419068 hasConcept C2780557392 @default.
• W2076419068 hasConcept C38441620 @default.
• W2076419068 hasConcept C41008148 @default.
• W2076419068 hasConcept C552990157 @default.
• W2076419068 hasConcept C55493867 @default.
• W2076419068 hasConcept C67705224 @default.
• W2076419068 hasConcept C71240020 @default.
• W2076419068 hasConcept C8010536 @default.
• W2076419068 hasConcept C99611785 @default.
• W2076419068 hasConceptScore W2076419068C104317684 @default.
• W2076419068 hasConceptScore W2076419068C111919701 @default.
• W2076419068 hasConceptScore W2076419068C111921641 @default.
• W2076419068 hasConceptScore W2076419068C129312508 @default.
• W2076419068 hasConceptScore W2076419068C185592680 @default.
• W2076419068 hasConceptScore W2076419068C21463570 @default.
• W2076419068 hasConceptScore W2076419068C2779321679 @default.
• W2076419068 hasConceptScore W2076419068C2780349520 @default.
• W2076419068 hasConceptScore W2076419068C2780557392 @default.
• W2076419068 hasConceptScore W2076419068C38441620 @default.
• W2076419068 hasConceptScore W2076419068C41008148 @default.
• W2076419068 hasConceptScore W2076419068C552990157 @default.
• W2076419068 hasConceptScore W2076419068C55493867 @default.
• W2076419068 hasConceptScore W2076419068C67705224 @default.

• next