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- W2076480212 abstract "A microtechnique for determining the superoxide dismutase activity in erythrocytes is described. This technique involves the inhibition of luminol-enhanced chemiluminescence of superoxide anion generated by xanthine-xanthine oxidase. Measurements required a steady-state chemiluminescence whether superoxide dismutase was present or absent; the level of luminescence was correlated to enzyme activity. Superoxide dismutase activity measured by this technique was 836 ± 112 μg/g of hemoglobin for whole blood and 834 ± 109 μg/g of hemoglobin for erythrocytes. When the reference technique was applied to larger amounts of blood, the results were 862 ± 58 and 858 ± 116 μg/g of hemoglobin for whole blood and washed erythrocytes, respectively. The enzymatic activity of superoxide dismutase from fetal blood (obtained by venipuncture in utero and of 19–26 weeks gestational age) was similar to that of adult blood, when measured by the new technique." @default.
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- W2076480212 date "1984-11-01" @default.
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- W2076480212 title "Luminol assay for microdetermination of superoxide dismutase activity: Its application to human fetal blood" @default.
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- W2076480212 doi "https://doi.org/10.1016/0003-2697(84)90491-3" @default.
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