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- W2076486098 abstract "Abstract Ampoules containing each 1 ml of Dulbecco's Modified Eagle Medium with 16.6% fetal calf serum and 10% dimethylsulfoxide were insulated in various ways and placed into different cooling boxes. The resulting cooling velocities of the medium ranged from about 0.7 to 102 °C/min. As revealed by cellular attachment in recovery cultures, human diploid fibroblasts cooled at about 1.5 to 4.5 °C/min to − 78 °C prior to storage in liquid nitrogen showed an optimal survival of about 60%. Survival was about 25% at cooling rates of 0.7 and 19 °C/min, respectively. The optimal cooling rate was achieved by insulating the freezing ampoules with 1 to 3 closed vessels and placing them into a dry ice chest, or into a dry ice/ethanol bath." @default.
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- W2076486098 date "1973-06-01" @default.
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- W2076486098 title "Studies on the optimal cooling rate for freezing human diploid fibroblasts" @default.
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- W2076486098 doi "https://doi.org/10.1016/0014-4827(73)90480-1" @default.
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