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• W2076495912 abstract "Abstract To determine if the ATP sulfurylase reaction is a regulatory step for the SO42−-assimilation pathway in plants, anArabidopsis thaliana ATP sulfurylase cDNA,APS2, was fused to the 35S promoter of the cauliflower mosaic virus and introduced by Agrobacterium tumefaciens-mediated transformation into isolated Bright Yellow 2 tobacco (Nicotiana tabacum) cells. The ATP sulfurylase activity in transgenic cells was 8-fold that in control cells, and was correlated with the expression of a specific polypeptide revealed by western analysis using an anti-ATP sulfurylase antibody. The molecular mass of this polypeptide agreed with that for the overexpressed mature protein. ATP sulfurylase overexpression had no effect on [35S]SO42− influx or ATP sulfurylase activity regulation by S availability, except that ATP sulfurylase activity variations in response to S starvation in transgenic cells were 8 times higher than in the wild type. There were also no differences in cell growth or sensitivity to SeO42− (a toxic SO42−analog) between transgenic and wild-type cells. We propose that in Bright Yellow 2 tobacco cells, the ATP sulfurylase derepression by S deficiency may involve a posttranscriptional mechanism, and that the ATP sulfurylase abundance is not limiting for cell metabolism." @default.
• W2076495912 created "2016-06-24" @default.
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• W2076495912 date "1998-04-01" @default.
• W2076495912 modified "2023-09-27" @default.
• W2076495912 title "Effect of ATP Sulfurylase Overexpression in Bright Yellow 2 Tobacco Cells1" @default.
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• W2076495912 doi "https://doi.org/10.1104/pp.116.4.1307" @default.
• W2076495912 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/35037" @default.
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