Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076525188> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2076525188 endingPage "581" @default.
- W2076525188 startingPage "577" @default.
- W2076525188 abstract "Northern corn leaf blight (NCLB) is a destructive foliar disease of maize that results from infection with the fungal pathogen, Exserohilum turcicum. Annual yield losses incurred from NCLB in South Africa may exceed 50 % when environmental conditions optimal for disease development prevail. In order to study the genetic diversity of E. turcicum, 13 microsatellite markers and mating type PCR primers were developed. Thirty-two primer pairs were designed from the E. turcicum genome sequence to flank microsatellite regions. A multiplex PCR assay amplifying both mating type idiomorphs was designed from the MAT1-1 and MAT1-2 gene sequences, and a protocol for multiplex PCR amplification of MAT loci was optimized. Initial screening identified 13 microsatellite regions that were polymorphic in 9 isolates of E. turcicum. To test the efficacy of the markers, 26 isolates of E. turcicum from 6 South African provinces, including 2 isolates from sorghum, were genotyped. A total of 90 alleles across 13 loci were obtained and the gene diversity ranged from 0.074 to 0.929. Cross-species amplification with E. rostratum was obtained for one SSR marker (SSR27). The MAT markers were specific to E. turcicum and could be used to differentiate isolates of E. turcicum and E. rostratum. The markers developed in this study will be useful to elucidate the population genetic structure, genetic diversity and mode of reproduction of E. turcicum on maize and sorghum." @default.
- W2076525188 created "2016-06-24" @default.
- W2076525188 creator A5009449442 @default.
- W2076525188 creator A5044749185 @default.
- W2076525188 creator A5058490973 @default.
- W2076525188 creator A5072102769 @default.
- W2076525188 date "2014-04-03" @default.
- W2076525188 modified "2023-09-24" @default.
- W2076525188 title "Microsatellite and mating type primers for the maize and sorghum pathogen, Exserohilum turcicum" @default.
- W2076525188 cites W1981670612 @default.
- W2076525188 cites W1988182864 @default.
- W2076525188 cites W1996201228 @default.
- W2076525188 cites W1998019829 @default.
- W2076525188 cites W2009823655 @default.
- W2076525188 cites W2010042331 @default.
- W2076525188 cites W2044129573 @default.
- W2076525188 cites W2082091267 @default.
- W2076525188 cites W2085044403 @default.
- W2076525188 cites W2095352800 @default.
- W2076525188 cites W2120644786 @default.
- W2076525188 cites W2128135663 @default.
- W2076525188 cites W2144924760 @default.
- W2076525188 cites W2158322738 @default.
- W2076525188 cites W2171787842 @default.
- W2076525188 cites W2314519588 @default.
- W2076525188 cites W2319391896 @default.
- W2076525188 cites W2333193738 @default.
- W2076525188 cites W2525224166 @default.
- W2076525188 cites W4254621947 @default.
- W2076525188 doi "https://doi.org/10.1007/s13313-014-0289-4" @default.
- W2076525188 hasPublicationYear "2014" @default.
- W2076525188 type Work @default.
- W2076525188 sameAs 2076525188 @default.
- W2076525188 citedByCount "14" @default.
- W2076525188 countsByYear W20765251882015 @default.
- W2076525188 countsByYear W20765251882016 @default.
- W2076525188 countsByYear W20765251882017 @default.
- W2076525188 countsByYear W20765251882018 @default.
- W2076525188 countsByYear W20765251882019 @default.
- W2076525188 countsByYear W20765251882020 @default.
- W2076525188 countsByYear W20765251882021 @default.
- W2076525188 countsByYear W20765251882022 @default.
- W2076525188 countsByYear W20765251882023 @default.
- W2076525188 crossrefType "journal-article" @default.
- W2076525188 hasAuthorship W2076525188A5009449442 @default.
- W2076525188 hasAuthorship W2076525188A5044749185 @default.
- W2076525188 hasAuthorship W2076525188A5058490973 @default.
- W2076525188 hasAuthorship W2076525188A5072102769 @default.
- W2076525188 hasBestOaLocation W20765251882 @default.
- W2076525188 hasConcept C104317684 @default.
- W2076525188 hasConcept C144024400 @default.
- W2076525188 hasConcept C149923435 @default.
- W2076525188 hasConcept C180754005 @default.
- W2076525188 hasConcept C182076605 @default.
- W2076525188 hasConcept C198348125 @default.
- W2076525188 hasConcept C2908647359 @default.
- W2076525188 hasConcept C2908728060 @default.
- W2076525188 hasConcept C54355233 @default.
- W2076525188 hasConcept C59822182 @default.
- W2076525188 hasConcept C61320498 @default.
- W2076525188 hasConcept C81977670 @default.
- W2076525188 hasConcept C86803240 @default.
- W2076525188 hasConceptScore W2076525188C104317684 @default.
- W2076525188 hasConceptScore W2076525188C144024400 @default.
- W2076525188 hasConceptScore W2076525188C149923435 @default.
- W2076525188 hasConceptScore W2076525188C180754005 @default.
- W2076525188 hasConceptScore W2076525188C182076605 @default.
- W2076525188 hasConceptScore W2076525188C198348125 @default.
- W2076525188 hasConceptScore W2076525188C2908647359 @default.
- W2076525188 hasConceptScore W2076525188C2908728060 @default.
- W2076525188 hasConceptScore W2076525188C54355233 @default.
- W2076525188 hasConceptScore W2076525188C59822182 @default.
- W2076525188 hasConceptScore W2076525188C61320498 @default.
- W2076525188 hasConceptScore W2076525188C81977670 @default.
- W2076525188 hasConceptScore W2076525188C86803240 @default.
- W2076525188 hasIssue "5" @default.
- W2076525188 hasLocation W20765251881 @default.
- W2076525188 hasLocation W20765251882 @default.
- W2076525188 hasOpenAccess W2076525188 @default.
- W2076525188 hasPrimaryLocation W20765251881 @default.
- W2076525188 hasRelatedWork W2058131830 @default.
- W2076525188 hasRelatedWork W2080288062 @default.
- W2076525188 hasRelatedWork W2153289919 @default.
- W2076525188 hasRelatedWork W226590832 @default.
- W2076525188 hasRelatedWork W2350309688 @default.
- W2076525188 hasRelatedWork W2560431690 @default.
- W2076525188 hasRelatedWork W2899201126 @default.
- W2076525188 hasRelatedWork W2991332150 @default.
- W2076525188 hasRelatedWork W3089227923 @default.
- W2076525188 hasRelatedWork W4221121552 @default.
- W2076525188 hasVolume "43" @default.
- W2076525188 isParatext "false" @default.
- W2076525188 isRetracted "false" @default.
- W2076525188 magId "2076525188" @default.
- W2076525188 workType "article" @default.