FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076532551> ?p ?o ?g. }

• W2076532551 endingPage "2974" @default.
• W2076532551 startingPage "2964" @default.
• W2076532551 abstract "Abstract Objective CD4+ T cells from patients with active lupus have impaired ERK pathway signaling that decreases DNA methyltransferase expression, resulting in DNA demethylation, overexpression of immune genes, and autoimmunity. The ERK pathway defect is due to impaired phosphorylation of T 505 in the protein kinase Cδ (PKCδ) activation loop. However, the mechanisms that prevent PKCδ T 505 phosphorylation in lupus T cells are unknown. Others have reported that oxidative modifications, and nitration in particular, of T cells as well as serum proteins correlate with lupus disease activity. We undertook this study to test our hypothesis that nitration inactivates PKCδ, contributing to impaired ERK pathway signaling in lupus T cells. Methods CD4+ T cells were purified from lupus patients and controls and then stimulated with phorbol myristate acetate (PMA). Signaling protein levels, nitration, and phosphorylation were quantitated by immunoprecipitation and immunoblotting of T cell lysates. Transfections were performed by electroporation. Results Treating CD4+ T cells with peroxynitrite nitrated PKCδ, preventing PKCδ T 505 phosphorylation and inhibiting ERK pathway signaling similar to that observed in lupus T cells. Patients with active lupus had higher nitrated T cell PKCδ levels than did controls, which correlated directly with disease activity, and antinitrotyrosine immunoprecipitations demonstrated that nitrated PKCδ, but not unmodified PKCδ, was refractory to PMA‐stimulated T 505 phosphorylation, similar to PKCδ in peroxynitrite‐treated cells. Conclusion Oxidative stress causes PKCδ nitration, which prevents its phosphorylation and contributes to the decreased ERK signaling in lupus T cells. These results identify PKCδ as a link between oxidative stress and the T cell epigenetic modifications in lupus." @default.
• W2076532551 created "2016-06-24" @default.
• W2076532551 creator A5013759503 @default.
• W2076532551 creator A5030199082 @default.
• W2076532551 creator A5045934111 @default.
• W2076532551 date "2012-08-27" @default.
• W2076532551 modified "2023-10-12" @default.
• W2076532551 title "Protein kinase Cδ oxidation contributes to ERK inactivation in lupus T cells" @default.
• W2076532551 cites W1541551778 @default.
• W2076532551 cites W1544893850 @default.
• W2076532551 cites W1576671418 @default.
• W2076532551 cites W1823692109 @default.
• W2076532551 cites W1970722898 @default.
• W2076532551 cites W1974429858 @default.
• W2076532551 cites W1982436260 @default.
• W2076532551 cites W1998349282 @default.
• W2076532551 cites W2006061640 @default.
• W2076532551 cites W2008088303 @default.
• W2076532551 cites W2008225234 @default.
• W2076532551 cites W2008261028 @default.
• W2076532551 cites W2009328811 @default.
• W2076532551 cites W2010897402 @default.
• W2076532551 cites W2011101614 @default.
• W2076532551 cites W2016957381 @default.
• W2076532551 cites W2017802764 @default.
• W2076532551 cites W2017870766 @default.
• W2076532551 cites W2020699105 @default.
• W2076532551 cites W2022051845 @default.
• W2076532551 cites W2022081039 @default.
• W2076532551 cites W2035672058 @default.
• W2076532551 cites W2044474149 @default.
• W2076532551 cites W2048132100 @default.
• W2076532551 cites W2063160236 @default.
• W2076532551 cites W2064999867 @default.
• W2076532551 cites W2065652048 @default.
• W2076532551 cites W2077863360 @default.
• W2076532551 cites W2080040722 @default.
• W2076532551 cites W2080145370 @default.
• W2076532551 cites W2083285984 @default.
• W2076532551 cites W2084410329 @default.
• W2076532551 cites W2087040058 @default.
• W2076532551 cites W2106309288 @default.
• W2076532551 cites W2118192449 @default.
• W2076532551 cites W2122386753 @default.
• W2076532551 cites W2123095447 @default.
• W2076532551 cites W2126681184 @default.
• W2076532551 cites W2134202806 @default.
• W2076532551 cites W2135015186 @default.
• W2076532551 cites W2137656282 @default.
• W2076532551 cites W2138825245 @default.
• W2076532551 cites W2143984422 @default.
• W2076532551 cites W2144556114 @default.
• W2076532551 cites W2146203649 @default.
• W2076532551 cites W2146989813 @default.
• W2076532551 cites W2156663501 @default.
• W2076532551 cites W2157296693 @default.
• W2076532551 cites W4237477922 @default.
• W2076532551 doi "https://doi.org/10.1002/art.34503" @default.
• W2076532551 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3414679" @default.
• W2076532551 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22549474" @default.
• W2076532551 hasPublicationYear "2012" @default.
• W2076532551 type Work @default.
• W2076532551 sameAs 2076532551 @default.
• W2076532551 citedByCount "55" @default.
• W2076532551 countsByYear W20765325512012 @default.
• W2076532551 countsByYear W20765325512013 @default.
• W2076532551 countsByYear W20765325512014 @default.
• W2076532551 countsByYear W20765325512015 @default.
• W2076532551 countsByYear W20765325512016 @default.
• W2076532551 countsByYear W20765325512017 @default.
• W2076532551 countsByYear W20765325512018 @default.
• W2076532551 countsByYear W20765325512019 @default.
• W2076532551 countsByYear W20765325512020 @default.
• W2076532551 countsByYear W20765325512021 @default.
• W2076532551 countsByYear W20765325512022 @default.
• W2076532551 countsByYear W20765325512023 @default.
• W2076532551 crossrefType "journal-article" @default.
• W2076532551 hasAuthorship W2076532551A5013759503 @default.
• W2076532551 hasAuthorship W2076532551A5030199082 @default.
• W2076532551 hasAuthorship W2076532551A5045934111 @default.
• W2076532551 hasBestOaLocation W20765325511 @default.
• W2076532551 hasConcept C11960822 @default.
• W2076532551 hasConcept C126322002 @default.
• W2076532551 hasConcept C153911025 @default.
• W2076532551 hasConcept C179464577 @default.
• W2076532551 hasConcept C185592680 @default.
• W2076532551 hasConcept C195794163 @default.
• W2076532551 hasConcept C203014093 @default.
• W2076532551 hasConcept C2776090121 @default.
• W2076532551 hasConcept C2776151105 @default.
• W2076532551 hasConcept C2776912625 @default.
• W2076532551 hasConcept C2779134260 @default.
• W2076532551 hasConcept C55493867 @default.
• W2076532551 hasConcept C57074206 @default.
• W2076532551 hasConcept C62478195 @default.
• W2076532551 hasConcept C71924100 @default.
• W2076532551 hasConcept C86803240 @default.
• W2076532551 hasConcept C8891405 @default.

• next