FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076533461> ?p ?o ?g. }

• W2076533461 endingPage "819" @default.
• W2076533461 startingPage "812" @default.
• W2076533461 abstract "Vascular injury and inflammation are associated with elaboration of a number of cytokines that signal through multiple pathways to act as smooth muscle cell (SMC) mitogens. Activation of the nuclear factor-kappa B (NF-kappaB) transcription factor is essential for SMC proliferation in vitro and is activated by vascular injury in vivo. Activation of NF-kappaB is controlled by several upstream regulators, including the inhibitors of kappa B (IkappaB). These proteins bind to and keep NF-kappaB inactivated. The purpose of this study was to determine whether adenoviral gene transfer of a mutated IkappaBalpha super-repressor (AdIkappaBalphaSR) could inhibit development of intimal hyperplasia in vivo and to investigate how over-expression of this construct influences in vitro SMC proliferation and cell cycle regulatory proteins.A rat carotid injury model was used to study prevention of intimal hyperplasia. Arteries were assayed 14 days after injury and infection with AdIkappaBalphaSR or adenoviral beta-galactosidase (AdLacZ). Untreated SMC or SMC infected with AdLacZ or AdIkappaBalphaSR were stimulated with 10% fetal bovine serum, interleukin-1beta, or tumor necrosis factor-alpha. Electrophoretic mobility shift assays were used to assay for NF-kappaB activation. Protein levels of IkappaBalpha and cyclin-dependent kinase inhibitors p21(Cip1/Waf1) and p27(Kip1) were determined with Western blot analysis. Proliferation was measured with (3)H-thymidine incorporation assays.AdIkappaBalphaSR inhibited the development of intimal hyperplasia by 49% (P <.05). Infection with AdIkappaBalphaSR significantly suppressed in vitro SMC proliferation when stimulated with serum, interleukin 1, or tumor necrosis factor alpha, and did not result in cell death. Inhibition of proliferation was associated with increased p21(Cip1/Waf1) and p27(Kip1) protein levels.Gene transfer of IkappaBalpha super-repressor inhibited development of intimal hyperplasia in vivo and SMC proliferation in vitro. The antiproliferative activity may be related to cell cycle arrest through upregulation of the cyclin-dependent kinase inhibitors p21 and p27. Overexpression of IkappaBalpha may be a future therapeutic option in treatment of vascular diseases." @default.
• W2076533461 created "2016-06-24" @default.
• W2076533461 creator A5002925691 @default.
• W2076533461 creator A5005725675 @default.
• W2076533461 creator A5038348270 @default.
• W2076533461 creator A5049195412 @default.
• W2076533461 creator A5061598858 @default.
• W2076533461 creator A5088355497 @default.
• W2076533461 date "2003-10-01" @default.
• W2076533461 modified "2023-09-28" @default.
• W2076533461 title "Overexpression of mutated IκBα inhibits vascular smooth muscle cell proliferation and intimal hyperplasia formation" @default.
• W2076533461 cites W1977909041 @default.
• W2076533461 cites W1982072699 @default.
• W2076533461 cites W1995203720 @default.
• W2076533461 cites W2020454682 @default.
• W2076533461 cites W2024185310 @default.
• W2076533461 cites W2024470090 @default.
• W2076533461 cites W2031629641 @default.
• W2076533461 cites W2060780133 @default.
• W2076533461 cites W2061512564 @default.
• W2076533461 cites W2066295010 @default.
• W2076533461 cites W2073032567 @default.
• W2076533461 cites W2079918729 @default.
• W2076533461 cites W2088848767 @default.
• W2076533461 cites W2124485728 @default.
• W2076533461 cites W2128494605 @default.
• W2076533461 cites W2152214792 @default.
• W2076533461 cites W2162748264 @default.
• W2076533461 cites W2166781237 @default.
• W2076533461 cites W2766221641 @default.
• W2076533461 cites W4313379449 @default.
• W2076533461 cites W71510850 @default.
• W2076533461 doi "https://doi.org/10.1016/s0741-5214(03)00427-0" @default.
• W2076533461 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14560235" @default.
• W2076533461 hasPublicationYear "2003" @default.
• W2076533461 type Work @default.
• W2076533461 sameAs 2076533461 @default.
• W2076533461 citedByCount "46" @default.
• W2076533461 countsByYear W20765334612012 @default.
• W2076533461 countsByYear W20765334612013 @default.
• W2076533461 countsByYear W20765334612016 @default.
• W2076533461 countsByYear W20765334612020 @default.
• W2076533461 countsByYear W20765334612021 @default.
• W2076533461 countsByYear W20765334612023 @default.
• W2076533461 crossrefType "journal-article" @default.
• W2076533461 hasAuthorship W2076533461A5002925691 @default.
• W2076533461 hasAuthorship W2076533461A5005725675 @default.
• W2076533461 hasAuthorship W2076533461A5038348270 @default.
• W2076533461 hasAuthorship W2076533461A5049195412 @default.
• W2076533461 hasAuthorship W2076533461A5061598858 @default.
• W2076533461 hasAuthorship W2076533461A5088355497 @default.
• W2076533461 hasBestOaLocation W20765334611 @default.
• W2076533461 hasConcept C126322002 @default.
• W2076533461 hasConcept C134018914 @default.
• W2076533461 hasConcept C142724271 @default.
• W2076533461 hasConcept C1491633281 @default.
• W2076533461 hasConcept C153911025 @default.
• W2076533461 hasConcept C17991360 @default.
• W2076533461 hasConcept C203014093 @default.
• W2076533461 hasConcept C2777339539 @default.
• W2076533461 hasConcept C2777562237 @default.
• W2076533461 hasConcept C2778095995 @default.
• W2076533461 hasConcept C2778283817 @default.
• W2076533461 hasConcept C2778583881 @default.
• W2076533461 hasConcept C2779395532 @default.
• W2076533461 hasConcept C2779404806 @default.
• W2076533461 hasConcept C2779751240 @default.
• W2076533461 hasConcept C2992686903 @default.
• W2076533461 hasConcept C502942594 @default.
• W2076533461 hasConcept C54355233 @default.
• W2076533461 hasConcept C62112901 @default.
• W2076533461 hasConcept C71924100 @default.
• W2076533461 hasConcept C86803240 @default.
• W2076533461 hasConcept C95444343 @default.
• W2076533461 hasConceptScore W2076533461C126322002 @default.
• W2076533461 hasConceptScore W2076533461C134018914 @default.
• W2076533461 hasConceptScore W2076533461C142724271 @default.
• W2076533461 hasConceptScore W2076533461C1491633281 @default.
• W2076533461 hasConceptScore W2076533461C153911025 @default.
• W2076533461 hasConceptScore W2076533461C17991360 @default.
• W2076533461 hasConceptScore W2076533461C203014093 @default.
• W2076533461 hasConceptScore W2076533461C2777339539 @default.
• W2076533461 hasConceptScore W2076533461C2777562237 @default.
• W2076533461 hasConceptScore W2076533461C2778095995 @default.
• W2076533461 hasConceptScore W2076533461C2778283817 @default.
• W2076533461 hasConceptScore W2076533461C2778583881 @default.
• W2076533461 hasConceptScore W2076533461C2779395532 @default.
• W2076533461 hasConceptScore W2076533461C2779404806 @default.
• W2076533461 hasConceptScore W2076533461C2779751240 @default.
• W2076533461 hasConceptScore W2076533461C2992686903 @default.
• W2076533461 hasConceptScore W2076533461C502942594 @default.
• W2076533461 hasConceptScore W2076533461C54355233 @default.
• W2076533461 hasConceptScore W2076533461C62112901 @default.
• W2076533461 hasConceptScore W2076533461C71924100 @default.
• W2076533461 hasConceptScore W2076533461C86803240 @default.
• W2076533461 hasConceptScore W2076533461C95444343 @default.
• W2076533461 hasIssue "4" @default.
• W2076533461 hasLocation W20765334611 @default.

• next