FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076539445> ?p ?o ?g. }

• W2076539445 endingPage "309" @default.
• W2076539445 startingPage "300" @default.
• W2076539445 abstract "A new method has been developed to accurately measure apurinic and apyrimidinic (AP) DNA damage sites, which are lesions in DNA formed by loss of a nucleobase from oxidative stress or carcinogen adducts. If AP sites are left unrepaired (or if improperly repaired), these sites can lead to DNA mutations that may ultimately result in the formation of cancer. Hence, detection of AP sites may provide a useful indicator of exposure and susceptibility to chemical carcinogens and oxidative stress. AP detection is currently accomplished by immunodetection methods using an aldehyde reactive probe [Nakamura, J., Walker, V. E., Upton, P. B., Chiang, S.-Y., Kow, Y. W., and Swenberg, J. A. (1998) Cancer Res. 58, 222-225; Atamna, H., Cheung, I., and Ames, B. N. (2000) Proc. Natl. Acad. Sci. U.S.A. 97, 686-691]; however, these approaches lack the specificity required for unequivocal identification of the AP site. Therefore, we have developed an accurate method based on mass spectrometry detection of AP sites from AP DNA that have been prelabeled with O-4-nitrobenzylhydroxylamine (NBHA). Once labeled and once the excess labeling agent has been removed, enzymatic digestion of DNA to monomeric subunits can be accomplished, followed by isolation and detection with high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS). Optimization and validation of the experimental procedures and detection limits have been established using a model DNA oligomer (11-mer) containing uracil. Enzymatic removal of uracil with uracil glycosylase generates well-defined AP sites in both single- and double-stranded DNA. The addition of NBHA labels the AP site in the oligomer, creating a labeled 11-mer. HPLC-ESI-MS/MS in the negative ionization mode was used to monitor and confirm binding of NBHA to the AP oligomer. The NBHA-tagged oligomer underwent endo- and exonuclease digestion to the 5'-deoxyribose monophosphate (5'-dRp) level, thereby releasing free 5'-dRp-NBHA. The 5'-dRp-NBHA product was partially purified by solid phase extraction and quantified by LC-MS/MS using several transitions of the deprotonated molecule ([M - H]- at m/z 363) and isotopically labeled 5'-dRp-NBHA as an internal standard. Further experiments with 5',3'-bisphosphate-deoxyribose and heat/acid-treated calf thymus DNA showed similar labeling, digestion, and detection results. Initial results show a quantification limit with 100 mug of DNA to be 100 fmol (three abasic sites per 10(7) bases)." @default.
• W2076539445 created "2016-06-24" @default.
• W2076539445 creator A5019121555 @default.
• W2076539445 creator A5032439241 @default.
• W2076539445 creator A5043108427 @default.
• W2076539445 creator A5052919605 @default.
• W2076539445 creator A5087972863 @default.
• W2076539445 date "2006-01-19" @default.
• W2076539445 modified "2023-10-16" @default.
• W2076539445 title "Determination of Apurinic/Apyrimidinic Lesions in DNA with High-Performance Liquid Chromatography and Tandem Mass Spectrometry" @default.
• W2076539445 cites W1986523486 @default.
• W2076539445 cites W2001757709 @default.
• W2076539445 cites W2003981071 @default.
• W2076539445 cites W2004144315 @default.
• W2076539445 cites W2023499540 @default.
• W2076539445 cites W2027116927 @default.
• W2076539445 cites W2042660284 @default.
• W2076539445 doi "https://doi.org/10.1021/tx0502589" @default.
• W2076539445 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16485907" @default.
• W2076539445 hasPublicationYear "2006" @default.
• W2076539445 type Work @default.
• W2076539445 sameAs 2076539445 @default.
• W2076539445 citedByCount "41" @default.
• W2076539445 countsByYear W20765394452012 @default.
• W2076539445 countsByYear W20765394452013 @default.
• W2076539445 countsByYear W20765394452014 @default.
• W2076539445 countsByYear W20765394452015 @default.
• W2076539445 countsByYear W20765394452016 @default.
• W2076539445 countsByYear W20765394452017 @default.
• W2076539445 countsByYear W20765394452018 @default.
• W2076539445 countsByYear W20765394452019 @default.
• W2076539445 countsByYear W20765394452020 @default.
• W2076539445 countsByYear W20765394452021 @default.
• W2076539445 countsByYear W20765394452022 @default.
• W2076539445 crossrefType "journal-article" @default.
• W2076539445 hasAuthorship W2076539445A5019121555 @default.
• W2076539445 hasAuthorship W2076539445A5032439241 @default.
• W2076539445 hasAuthorship W2076539445A5043108427 @default.
• W2076539445 hasAuthorship W2076539445A5052919605 @default.
• W2076539445 hasAuthorship W2076539445A5087972863 @default.
• W2076539445 hasConcept C114246631 @default.
• W2076539445 hasConcept C143425029 @default.
• W2076539445 hasConcept C150777479 @default.
• W2076539445 hasConcept C162356407 @default.
• W2076539445 hasConcept C185592680 @default.
• W2076539445 hasConcept C192396546 @default.
• W2076539445 hasConcept C31827203 @default.
• W2076539445 hasConcept C40684141 @default.
• W2076539445 hasConcept C43617362 @default.
• W2076539445 hasConcept C552990157 @default.
• W2076539445 hasConcept C55493867 @default.
• W2076539445 hasConceptScore W2076539445C114246631 @default.
• W2076539445 hasConceptScore W2076539445C143425029 @default.
• W2076539445 hasConceptScore W2076539445C150777479 @default.
• W2076539445 hasConceptScore W2076539445C162356407 @default.
• W2076539445 hasConceptScore W2076539445C185592680 @default.
• W2076539445 hasConceptScore W2076539445C192396546 @default.
• W2076539445 hasConceptScore W2076539445C31827203 @default.
• W2076539445 hasConceptScore W2076539445C40684141 @default.
• W2076539445 hasConceptScore W2076539445C43617362 @default.
• W2076539445 hasConceptScore W2076539445C552990157 @default.
• W2076539445 hasConceptScore W2076539445C55493867 @default.
• W2076539445 hasIssue "2" @default.
• W2076539445 hasLocation W20765394451 @default.
• W2076539445 hasLocation W20765394452 @default.
• W2076539445 hasOpenAccess W2076539445 @default.
• W2076539445 hasPrimaryLocation W20765394451 @default.
• W2076539445 hasRelatedWork W2031101499 @default.
• W2076539445 hasRelatedWork W2063163055 @default.
• W2076539445 hasRelatedWork W2068220611 @default.
• W2076539445 hasRelatedWork W2144712334 @default.
• W2076539445 hasRelatedWork W2168882862 @default.
• W2076539445 hasRelatedWork W2528508621 @default.
• W2076539445 hasRelatedWork W4231593990 @default.
• W2076539445 hasRelatedWork W4378835358 @default.
• W2076539445 hasRelatedWork W4380992534 @default.
• W2076539445 hasRelatedWork W53635053 @default.
• W2076539445 hasVolume "19" @default.
• W2076539445 isParatext "false" @default.
• W2076539445 isRetracted "false" @default.
• W2076539445 magId "2076539445" @default.
• W2076539445 workType "article" @default.