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- W2076548710 abstract "The synthesis of the macrocyclic rhodamine 110 caspase 3 substrate 8 is described. The key step is a high dilution intramolecular cyclization reaction of an in situ generated primary amine with a 4-nitrophenyl ester. Substrate 8 reacts with recombinant caspase 3 to yield a fluorescent signal but virtually no signal is detected in the absence of caspase 3 or in the presence of the caspase 3 inhibitor Ac-DEVD-CHO. Notably, 8 selectively stains live cells that have been induced to undergo apoptosis with etoposide." @default.
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- W2076548710 date "2000-06-01" @default.
- W2076548710 modified "2023-09-26" @default.
- W2076548710 title "Synthesis of a macrocyclic rhodamine 110 enzyme substrate as an intracellular probe for caspase 3 activity" @default.
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- W2076548710 doi "https://doi.org/10.1016/s0040-4039(00)00708-5" @default.
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