FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076553464> ?p ?o ?g. }

• W2076553464 endingPage "776" @default.
• W2076553464 startingPage "769" @default.
• W2076553464 abstract "The biochemical transductional events involved in NO synthesis are not fully understood. These studies, therefore, were undertaken to elucidate the role of intracellular calcium and protein kinase C (PKC) in the induction of nitric oxide (NO) synthesis in murine peritoneal macrophages. Thapsigargin (TG), Ca(2+)-ATPase inhibitor of endoplasmic reticulum, had modest activity on NO synthesis by itself, whereas phorbol ester, PKC activator, alone had no effect. When TG was used in combination with phorbol ester, there was a marked cooperative induction of NO synthesis in a dose-dependent manner. The optimal effect of phorbol ester was shown in the first 6 h after TG treatment. In addition, the ability of TG with phorbol ester on NO synthesis could be mimicked by another chemically unrelated inhibitor of Ca(2+)-ATPase, 2,5-di-(t-butyl)-1, 4-benzohydroquinone, and Ca2+ ionophore, A23187. This increase of NO synthesis was reflected as increased amount of NO synthase (NOS) mRNA, as determined by Northern blotting. Intracellular Ca2+ transient by TG was not affected in the presence or absence of extracellular Ca2+, indicating that TG must be effective on cytosolic Ca2+ pool. In addition, chelation of intracellular Ca2+ by acetoxymethyl ester of 1,2-bis-(2-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid (BAPTA/AM), an intracellular Ca2+ chelating agent, blocked TG- or TG + PMA-induced NO production. PKC inhibitors such as staurosporine or polymyxin B reduced only the synergistic cooperative effect of TG with phorbol ester without affecting TG-induced NO production. In addition, when the cells were pretreated with phorbol ester before TG treatment, there was no synergy between TG and phorbol ester, indicating that PKC is not directly involved in the expression of NOS but involved in triggering signal. Secretion of NO corresponded with tumor cell killing, but TG plus phorbol ester-activated macrophages failed to kill tumor cell targets in the presence of Ng-monomethyl-L-arginine. Collectively, these data illustrate that mobilization of intracellular Ca2+ provides a priming signal for induction of NOS gene expression by itself and it also requires PKC as a triggering signal for macrophage tumoricidal activity." @default.
• W2076553464 created "2016-06-24" @default.
• W2076553464 creator A5020898882 @default.
• W2076553464 creator A5023173703 @default.
• W2076553464 creator A5034003059 @default.
• W2076553464 creator A5046879145 @default.
• W2076553464 creator A5051192204 @default.
• W2076553464 creator A5052488200 @default.
• W2076553464 creator A5090068470 @default.
• W2076553464 date "1996-01-01" @default.
• W2076553464 modified "2023-10-16" @default.
• W2076553464 title "Synergistic cooperation between thapsigargin and phorbol ester for induction of nitric oxide synthesis in murine peritoneal macrophages" @default.
• W2076553464 cites W1496717884 @default.
• W2076553464 cites W1496997991 @default.
• W2076553464 cites W1509810344 @default.
• W2076553464 cites W1600116708 @default.
• W2076553464 cites W1605949901 @default.
• W2076553464 cites W1664175075 @default.
• W2076553464 cites W1765098545 @default.
• W2076553464 cites W1990205754 @default.
• W2076553464 cites W1990802251 @default.
• W2076553464 cites W1994303699 @default.
• W2076553464 cites W1994389040 @default.
• W2076553464 cites W2004734553 @default.
• W2076553464 cites W2013114215 @default.
• W2076553464 cites W2023170094 @default.
• W2076553464 cites W2060333964 @default.
• W2076553464 cites W2077538369 @default.
• W2076553464 cites W2127457813 @default.
• W2076553464 cites W2169801629 @default.
• W2076553464 cites W2396028691 @default.
• W2076553464 cites W2397741376 @default.
• W2076553464 cites W2434348074 @default.
• W2076553464 doi "https://doi.org/10.1016/0891-5849(95)02157-4" @default.
• W2076553464 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8728023" @default.
• W2076553464 hasPublicationYear "1996" @default.
• W2076553464 type Work @default.
• W2076553464 sameAs 2076553464 @default.
• W2076553464 citedByCount "11" @default.
• W2076553464 countsByYear W20765534642016 @default.
• W2076553464 countsByYear W20765534642021 @default.
• W2076553464 crossrefType "journal-article" @default.
• W2076553464 hasAuthorship W2076553464A5020898882 @default.
• W2076553464 hasAuthorship W2076553464A5023173703 @default.
• W2076553464 hasAuthorship W2076553464A5034003059 @default.
• W2076553464 hasAuthorship W2076553464A5046879145 @default.
• W2076553464 hasAuthorship W2076553464A5051192204 @default.
• W2076553464 hasAuthorship W2076553464A5052488200 @default.
• W2076553464 hasAuthorship W2076553464A5090068470 @default.
• W2076553464 hasConcept C153911025 @default.
• W2076553464 hasConcept C158617107 @default.
• W2076553464 hasConcept C170493617 @default.
• W2076553464 hasConcept C178790620 @default.
• W2076553464 hasConcept C181080969 @default.
• W2076553464 hasConcept C181199279 @default.
• W2076553464 hasConcept C184235292 @default.
• W2076553464 hasConcept C185592680 @default.
• W2076553464 hasConcept C195794163 @default.
• W2076553464 hasConcept C2777146706 @default.
• W2076553464 hasConcept C2777622882 @default.
• W2076553464 hasConcept C2779084600 @default.
• W2076553464 hasConcept C2780019300 @default.
• W2076553464 hasConcept C2780043322 @default.
• W2076553464 hasConcept C28406088 @default.
• W2076553464 hasConcept C519581460 @default.
• W2076553464 hasConcept C55493867 @default.
• W2076553464 hasConcept C79879829 @default.
• W2076553464 hasConcept C86803240 @default.
• W2076553464 hasConcept C88045685 @default.
• W2076553464 hasConceptScore W2076553464C153911025 @default.
• W2076553464 hasConceptScore W2076553464C158617107 @default.
• W2076553464 hasConceptScore W2076553464C170493617 @default.
• W2076553464 hasConceptScore W2076553464C178790620 @default.
• W2076553464 hasConceptScore W2076553464C181080969 @default.
• W2076553464 hasConceptScore W2076553464C181199279 @default.
• W2076553464 hasConceptScore W2076553464C184235292 @default.
• W2076553464 hasConceptScore W2076553464C185592680 @default.
• W2076553464 hasConceptScore W2076553464C195794163 @default.
• W2076553464 hasConceptScore W2076553464C2777146706 @default.
• W2076553464 hasConceptScore W2076553464C2777622882 @default.
• W2076553464 hasConceptScore W2076553464C2779084600 @default.
• W2076553464 hasConceptScore W2076553464C2780019300 @default.
• W2076553464 hasConceptScore W2076553464C2780043322 @default.
• W2076553464 hasConceptScore W2076553464C28406088 @default.
• W2076553464 hasConceptScore W2076553464C519581460 @default.
• W2076553464 hasConceptScore W2076553464C55493867 @default.
• W2076553464 hasConceptScore W2076553464C79879829 @default.
• W2076553464 hasConceptScore W2076553464C86803240 @default.
• W2076553464 hasConceptScore W2076553464C88045685 @default.
• W2076553464 hasIssue "6" @default.
• W2076553464 hasLocation W20765534641 @default.
• W2076553464 hasLocation W20765534642 @default.
• W2076553464 hasOpenAccess W2076553464 @default.
• W2076553464 hasPrimaryLocation W20765534641 @default.
• W2076553464 hasRelatedWork W1966254317 @default.
• W2076553464 hasRelatedWork W2001319577 @default.
• W2076553464 hasRelatedWork W2047710228 @default.
• W2076553464 hasRelatedWork W2058180049 @default.

• next