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• W2076561679 startingPage "1796" @default.
• W2076561679 abstract "The atomic force microscope is a useful tool for imaging native biological structures at high resolution. In analogy to conventional immunolabeling techniques, we have used antibodies directed against the C-terminus of bacteriorhodopsin to distinguish the cytoplasmic and extracellular surface of purple membrane while imaging in buffer solution. At forces > or = 0.8 nN the antibodies were removed by the scanning stylus and the molecular topography of the cytoplasmic purple membrane surface was revealed. When the stylus was retracted, the scanned membrane area was relabeled with antibodies within 10 min. The extracellular surface of purple membrane was imaged at 0.7 nm resolution, exhibiting a major and a minor protrusion per bacteriorhodopsin monomer. As confirmed by immuno-dot blot analysis and sodium dodecyl sulfate-gel electrophoresis, labeling of the purple membrane was not observed if the C-terminus of bacteriorhodopsin was cleaved off by papain." @default.
• W2076561679 created "2016-06-24" @default.
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• W2076561679 date "1996-04-01" @default.
• W2076561679 modified "2023-09-28" @default.
• W2076561679 title "Immuno-atomic force microscopy of purple membrane" @default.
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• W2076561679 doi "https://doi.org/10.1016/s0006-3495(96)79743-6" @default.
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