FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076571844> ?p ?o ?g. }

• W2076571844 endingPage "386" @default.
• W2076571844 startingPage "377" @default.
• W2076571844 abstract "Physical stability is critical for any therapeutic protein's efficacy and economic viability. No reliable theory exists to predict stability de novo, and modeling aggregation is challenging as this phenomenon can involve orientation effects, unfolding, and the rearrangement of noncovalent bonds inter- and intramolecularly in a complex sequence of poorly understood events. Despite this complexity, the simple observation of protein concentration-dependent diffusivity in stable, low ionic-strength solutions can provide valuable information about a protein's propensity to aggregate at higher salt concentrations and over longer times. We recently verified this notion using two model proteins, and others have shown that this strategy may be applicable to antibodies as well. Here, we expand our previous study to a monoclonal human immunoglobulin G1 antibody and discuss both merits and limitations of stability assessments based on the diffusional virial coefficient kD. We find this parameter to be a good predictor of relative protein stability in solutions of different chaotropic salts, and a telling heuristic for the effect of kosmotropes. Both temperature and glycosylation are seen to have a strong influence on kD, and we examine how these factors affect stability assessments. Protein unfolding is monitored with a fluorescence assay to assist in interpreting the observed aggregation rates. © 2012 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:377–386, 2013" @default.
• W2076571844 created "2016-06-24" @default.
• W2076571844 creator A5000034827 @default.
• W2076571844 creator A5026009346 @default.
• W2076571844 creator A5055976215 @default.
• W2076571844 creator A5074292836 @default.
• W2076571844 creator A5080348602 @default.
• W2076571844 date "2013-02-01" @default.
• W2076571844 modified "2023-09-26" @default.
• W2076571844 title "Salt-Induced Aggregation of a Monoclonal Human Immunoglobulin G1" @default.
• W2076571844 cites W1581858536 @default.
• W2076571844 cites W195296421 @default.
• W2076571844 cites W1980195682 @default.
• W2076571844 cites W1985181309 @default.
• W2076571844 cites W1988834123 @default.
• W2076571844 cites W1991163974 @default.
• W2076571844 cites W1995692089 @default.
• W2076571844 cites W2015799566 @default.
• W2076571844 cites W2023754316 @default.
• W2076571844 cites W2024304683 @default.
• W2076571844 cites W2024641545 @default.
• W2076571844 cites W2028928132 @default.
• W2076571844 cites W2029314929 @default.
• W2076571844 cites W2031691673 @default.
• W2076571844 cites W2036879738 @default.
• W2076571844 cites W2038810416 @default.
• W2076571844 cites W2038968935 @default.
• W2076571844 cites W2040660378 @default.
• W2076571844 cites W2050754173 @default.
• W2076571844 cites W2057204174 @default.
• W2076571844 cites W2069616361 @default.
• W2076571844 cites W2071095038 @default.
• W2076571844 cites W2077069163 @default.
• W2076571844 cites W2083531736 @default.
• W2076571844 cites W2090690774 @default.
• W2076571844 cites W2091714383 @default.
• W2076571844 cites W2093677418 @default.
• W2076571844 cites W2095256587 @default.
• W2076571844 cites W2096785891 @default.
• W2076571844 cites W2097421000 @default.
• W2076571844 cites W2123331319 @default.
• W2076571844 cites W2145538573 @default.
• W2076571844 cites W2154280514 @default.
• W2076571844 cites W2160813631 @default.
• W2076571844 cites W4252254711 @default.
• W2076571844 doi "https://doi.org/10.1002/jps.23363" @default.
• W2076571844 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23147563" @default.
• W2076571844 hasPublicationYear "2013" @default.
• W2076571844 type Work @default.
• W2076571844 sameAs 2076571844 @default.
• W2076571844 citedByCount "29" @default.
• W2076571844 countsByYear W20765718442013 @default.
• W2076571844 countsByYear W20765718442014 @default.
• W2076571844 countsByYear W20765718442015 @default.
• W2076571844 countsByYear W20765718442016 @default.
• W2076571844 countsByYear W20765718442017 @default.
• W2076571844 countsByYear W20765718442018 @default.
• W2076571844 countsByYear W20765718442019 @default.
• W2076571844 countsByYear W20765718442020 @default.
• W2076571844 countsByYear W20765718442021 @default.
• W2076571844 countsByYear W20765718442022 @default.
• W2076571844 countsByYear W20765718442023 @default.
• W2076571844 crossrefType "journal-article" @default.
• W2076571844 hasAuthorship W2076571844A5000034827 @default.
• W2076571844 hasAuthorship W2076571844A5026009346 @default.
• W2076571844 hasAuthorship W2076571844A5055976215 @default.
• W2076571844 hasAuthorship W2076571844A5074292836 @default.
• W2076571844 hasAuthorship W2076571844A5080348602 @default.
• W2076571844 hasConcept C102362939 @default.
• W2076571844 hasConcept C12554922 @default.
• W2076571844 hasConcept C136238340 @default.
• W2076571844 hasConcept C147789679 @default.
• W2076571844 hasConcept C159654299 @default.
• W2076571844 hasConcept C166940927 @default.
• W2076571844 hasConcept C178790620 @default.
• W2076571844 hasConcept C184651966 @default.
• W2076571844 hasConcept C185592680 @default.
• W2076571844 hasConcept C187428577 @default.
• W2076571844 hasConcept C203014093 @default.
• W2076571844 hasConcept C2776371256 @default.
• W2076571844 hasConcept C2988375501 @default.
• W2076571844 hasConcept C521977710 @default.
• W2076571844 hasConcept C542903549 @default.
• W2076571844 hasConcept C55493867 @default.
• W2076571844 hasConcept C86803240 @default.
• W2076571844 hasConceptScore W2076571844C102362939 @default.
• W2076571844 hasConceptScore W2076571844C12554922 @default.
• W2076571844 hasConceptScore W2076571844C136238340 @default.
• W2076571844 hasConceptScore W2076571844C147789679 @default.
• W2076571844 hasConceptScore W2076571844C159654299 @default.
• W2076571844 hasConceptScore W2076571844C166940927 @default.
• W2076571844 hasConceptScore W2076571844C178790620 @default.
• W2076571844 hasConceptScore W2076571844C184651966 @default.
• W2076571844 hasConceptScore W2076571844C185592680 @default.
• W2076571844 hasConceptScore W2076571844C187428577 @default.
• W2076571844 hasConceptScore W2076571844C203014093 @default.
• W2076571844 hasConceptScore W2076571844C2776371256 @default.
• W2076571844 hasConceptScore W2076571844C2988375501 @default.

• next