FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076574570> ?p ?o ?g. }

• W2076574570 abstract "The P22 tailspike adhesin is an elongated thermostable trimer resistant to protease digestion and to denaturation in sodium dodecyl sulfate. Monomeric, dimeric, and protrimeric folding and assembly intermediates lack this stability and are thermolabile. In the native trimer, three right-handed parallel beta-helices (residues 143-540), pack side-by-side around the three-fold axis. After residue 540, these single chain beta-helices terminate and residues 541-567 of the three polypeptide chains wrap around each other to form a three-stranded interdigitated beta-helix. Three mutants located in this region -- G546D, R563Q, and A575T -- blocked formation of native tailspike trimers, and accumulated soluble forms of the mutant polypeptide chains within cells. The substitutions R563Q and A575T appeared to prevent stable association of partially folded monomers. G546D, in the interdigitated region of the chain, blocked tailspike folding at the transition from the partially-folded protrimer to the native trimer. The protrimer-like species accumulating in the G546D mutant melted out at 42 degrees C and was trypsin and SDS sensitive. The G546D defect was not corrected by introduction of global suppressor mutations, which correct kinetic defects in beta-helix folding. The simplest interpretation of these results is that the very high thermostability (T(m) = 88 degrees C), protease and detergent resistance of the native tailspike acquired in the protrimer-to-trimer transition, depends on the formation of the three-stranded interdigitated region. This interdigitated beta-helix appears to function as a molecular clamp insuring thermostable subunit association in the native trimer." @default.
• W2076574570 created "2016-06-24" @default.
• W2076574570 creator A5002701325 @default.
• W2076574570 creator A5023368909 @default.
• W2076574570 creator A5054662306 @default.
• W2076574570 creator A5084191502 @default.
• W2076574570 date "2002-04-01" @default.
• W2076574570 modified "2023-09-24" @default.
• W2076574570 title "The interdigitated beta-helix domain of the P22 tailspike protein acts as a molecular clamp in trimer stabilization" @default.
• W2076574570 cites W1481936182 @default.
• W2076574570 cites W1487467326 @default.
• W2076574570 cites W1489708565 @default.
• W2076574570 cites W1517176127 @default.
• W2076574570 cites W1551315742 @default.
• W2076574570 cites W1570203195 @default.
• W2076574570 cites W1590313270 @default.
• W2076574570 cites W1591847193 @default.
• W2076574570 cites W1594450556 @default.
• W2076574570 cites W1597218599 @default.
• W2076574570 cites W1608094563 @default.
• W2076574570 cites W1647904924 @default.
• W2076574570 cites W1980597219 @default.
• W2076574570 cites W1985899278 @default.
• W2076574570 cites W1988814356 @default.
• W2076574570 cites W1989073611 @default.
• W2076574570 cites W1996885522 @default.
• W2076574570 cites W2001850081 @default.
• W2076574570 cites W2003459056 @default.
• W2076574570 cites W2007171153 @default.
• W2076574570 cites W2009292400 @default.
• W2076574570 cites W2009800352 @default.
• W2076574570 cites W2026141408 @default.
• W2076574570 cites W2026346121 @default.
• W2076574570 cites W2030093817 @default.
• W2076574570 cites W2037109486 @default.
• W2076574570 cites W2043054178 @default.
• W2076574570 cites W2045476844 @default.
• W2076574570 cites W2047308848 @default.
• W2076574570 cites W2049885305 @default.
• W2076574570 cites W2052003358 @default.
• W2076574570 cites W2054441789 @default.
• W2076574570 cites W2058381769 @default.
• W2076574570 cites W2074219304 @default.
• W2076574570 cites W2079010479 @default.
• W2076574570 cites W2082968224 @default.
• W2076574570 cites W2100390700 @default.
• W2076574570 cites W2112129918 @default.
• W2076574570 cites W2112501830 @default.
• W2076574570 cites W2112879903 @default.
• W2076574570 cites W2115453381 @default.
• W2076574570 cites W2141260048 @default.
• W2076574570 cites W2144712088 @default.
• W2076574570 cites W2155837025 @default.
• W2076574570 cites W2157644461 @default.
• W2076574570 cites W2167139715 @default.
• W2076574570 cites W2171343897 @default.
• W2076574570 cites W2108242612 @default.
• W2076574570 doi "https://doi.org/10.1110/ps.3440102" @default.
• W2076574570 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2373520" @default.
• W2076574570 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11910025" @default.
• W2076574570 hasPublicationYear "2002" @default.
• W2076574570 type Work @default.
• W2076574570 sameAs 2076574570 @default.
• W2076574570 citedByCount "32" @default.
• W2076574570 countsByYear W20765745702012 @default.
• W2076574570 countsByYear W20765745702013 @default.
• W2076574570 countsByYear W20765745702015 @default.
• W2076574570 countsByYear W20765745702017 @default.
• W2076574570 countsByYear W20765745702019 @default.
• W2076574570 crossrefType "journal-article" @default.
• W2076574570 hasAuthorship W2076574570A5002701325 @default.
• W2076574570 hasAuthorship W2076574570A5023368909 @default.
• W2076574570 hasAuthorship W2076574570A5054662306 @default.
• W2076574570 hasAuthorship W2076574570A5084191502 @default.
• W2076574570 hasBestOaLocation W20765745702 @default.
• W2076574570 hasConcept C104317684 @default.
• W2076574570 hasConcept C119599485 @default.
• W2076574570 hasConcept C12554922 @default.
• W2076574570 hasConcept C127413603 @default.
• W2076574570 hasConcept C13965031 @default.
• W2076574570 hasConcept C143065580 @default.
• W2076574570 hasConcept C166940927 @default.
• W2076574570 hasConcept C178790620 @default.
• W2076574570 hasConcept C185592680 @default.
• W2076574570 hasConcept C18903297 @default.
• W2076574570 hasConcept C204328495 @default.
• W2076574570 hasConcept C2776403692 @default.
• W2076574570 hasConcept C2776545253 @default.
• W2076574570 hasConcept C2776923230 @default.
• W2076574570 hasConcept C2778530040 @default.
• W2076574570 hasConcept C2779546866 @default.
• W2076574570 hasConcept C2779965526 @default.
• W2076574570 hasConcept C47701112 @default.
• W2076574570 hasConcept C521977710 @default.
• W2076574570 hasConcept C55493867 @default.
• W2076574570 hasConcept C71240020 @default.
• W2076574570 hasConcept C8010536 @default.
• W2076574570 hasConcept C86803240 @default.
• W2076574570 hasConceptScore W2076574570C104317684 @default.
• W2076574570 hasConceptScore W2076574570C119599485 @default.

• next