Matches in SemOpenAlex for { <https://semopenalex.org/work/W2076656110> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2076656110 endingPage "70" @default.
- W2076656110 startingPage "62" @default.
- W2076656110 abstract "A chip-based biosensor technology using surface plasmon resonance (SPR) was developed for studying the interaction of ligands and G protein-coupled receptors (GPCRs). GPCRs, the fourth largest superfamily in the human genome, are the largest class of targets for drug discovery. We have expressed the three subtypes of α2-adrenergic receptor (α2-AR), a prototypical GPCR as functional fusion proteins in baculovirus-infected insect cells. The localization of the expressed receptor was observed in intracellular organelles, as detected by eGFP fluorescence. In addition, the deletion mutants of α2B-AR, with a deletion in the 3rd intracellular loop, exhibited unaltered Kd values and enhanced stability, thus making them more promising candidates for crystallization. SPR demonstrated that small molecule ligands can bind the detergent-solubilized receptor, thus proving that α2-AR is active even in a lipid-free environment. The Kd values obtained from the biosensor analysis and traditional ligand binding studies correlate well with each other. This is the first demonstration of the binding of a small molecule to the detergent-solubilized state of α2-ARs and interaction of low-molecular mass-ligands in real time in a label-free environment. This technology will also allow the development of high throughput platform for screening a large number of compounds for generation of leads." @default.
- W2076656110 created "2016-06-24" @default.
- W2076656110 creator A5000684750 @default.
- W2076656110 creator A5023382504 @default.
- W2076656110 creator A5026551332 @default.
- W2076656110 creator A5065316967 @default.
- W2076656110 creator A5074933688 @default.
- W2076656110 date "2005-06-01" @default.
- W2076656110 modified "2023-10-12" @default.
- W2076656110 title "Functional studies with membrane-bound and detergent-solubilized α2-adrenergic receptors expressed in Sf9 cells" @default.
- W2076656110 cites W1602157168 @default.
- W2076656110 cites W1965262011 @default.
- W2076656110 cites W1971886200 @default.
- W2076656110 cites W1996568268 @default.
- W2076656110 cites W2000262653 @default.
- W2076656110 cites W2002733371 @default.
- W2076656110 cites W2006913937 @default.
- W2076656110 cites W2009133356 @default.
- W2076656110 cites W2022162177 @default.
- W2076656110 cites W2023537796 @default.
- W2076656110 cites W2044975682 @default.
- W2076656110 cites W2050517052 @default.
- W2076656110 cites W2063998199 @default.
- W2076656110 cites W2065954013 @default.
- W2076656110 cites W2087172623 @default.
- W2076656110 cites W2088157575 @default.
- W2076656110 cites W2091933223 @default.
- W2076656110 cites W2100837269 @default.
- W2076656110 cites W2101108802 @default.
- W2076656110 cites W2156294304 @default.
- W2076656110 doi "https://doi.org/10.1016/j.bbamem.2005.03.014" @default.
- W2076656110 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15893292" @default.
- W2076656110 hasPublicationYear "2005" @default.
- W2076656110 type Work @default.
- W2076656110 sameAs 2076656110 @default.
- W2076656110 citedByCount "26" @default.
- W2076656110 countsByYear W20766561102013 @default.
- W2076656110 countsByYear W20766561102014 @default.
- W2076656110 countsByYear W20766561102015 @default.
- W2076656110 crossrefType "journal-article" @default.
- W2076656110 hasAuthorship W2076656110A5000684750 @default.
- W2076656110 hasAuthorship W2076656110A5023382504 @default.
- W2076656110 hasAuthorship W2076656110A5026551332 @default.
- W2076656110 hasAuthorship W2076656110A5065316967 @default.
- W2076656110 hasAuthorship W2076656110A5074933688 @default.
- W2076656110 hasBestOaLocation W20766561101 @default.
- W2076656110 hasConcept C106847996 @default.
- W2076656110 hasConcept C116569031 @default.
- W2076656110 hasConcept C12554922 @default.
- W2076656110 hasConcept C135285700 @default.
- W2076656110 hasConcept C155672457 @default.
- W2076656110 hasConcept C161624437 @default.
- W2076656110 hasConcept C168240541 @default.
- W2076656110 hasConcept C170493617 @default.
- W2076656110 hasConcept C171250308 @default.
- W2076656110 hasConcept C185592680 @default.
- W2076656110 hasConcept C192562407 @default.
- W2076656110 hasConcept C55493867 @default.
- W2076656110 hasConcept C74187038 @default.
- W2076656110 hasConcept C79879829 @default.
- W2076656110 hasConcept C86803240 @default.
- W2076656110 hasConcept C95444343 @default.
- W2076656110 hasConceptScore W2076656110C106847996 @default.
- W2076656110 hasConceptScore W2076656110C116569031 @default.
- W2076656110 hasConceptScore W2076656110C12554922 @default.
- W2076656110 hasConceptScore W2076656110C135285700 @default.
- W2076656110 hasConceptScore W2076656110C155672457 @default.
- W2076656110 hasConceptScore W2076656110C161624437 @default.
- W2076656110 hasConceptScore W2076656110C168240541 @default.
- W2076656110 hasConceptScore W2076656110C170493617 @default.
- W2076656110 hasConceptScore W2076656110C171250308 @default.
- W2076656110 hasConceptScore W2076656110C185592680 @default.
- W2076656110 hasConceptScore W2076656110C192562407 @default.
- W2076656110 hasConceptScore W2076656110C55493867 @default.
- W2076656110 hasConceptScore W2076656110C74187038 @default.
- W2076656110 hasConceptScore W2076656110C79879829 @default.
- W2076656110 hasConceptScore W2076656110C86803240 @default.
- W2076656110 hasConceptScore W2076656110C95444343 @default.
- W2076656110 hasIssue "1" @default.
- W2076656110 hasLocation W20766561101 @default.
- W2076656110 hasLocation W20766561102 @default.
- W2076656110 hasOpenAccess W2076656110 @default.
- W2076656110 hasPrimaryLocation W20766561101 @default.
- W2076656110 hasRelatedWork W1974664442 @default.
- W2076656110 hasRelatedWork W2019365911 @default.
- W2076656110 hasRelatedWork W2114330152 @default.
- W2076656110 hasRelatedWork W2168448982 @default.
- W2076656110 hasRelatedWork W2401714899 @default.
- W2076656110 hasRelatedWork W263755546 @default.
- W2076656110 hasRelatedWork W2797401814 @default.
- W2076656110 hasRelatedWork W2807951268 @default.
- W2076656110 hasRelatedWork W2893816517 @default.
- W2076656110 hasRelatedWork W3112815829 @default.
- W2076656110 hasVolume "1712" @default.
- W2076656110 isParatext "false" @default.
- W2076656110 isRetracted "false" @default.
- W2076656110 magId "2076656110" @default.
- W2076656110 workType "article" @default.