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- W2076683313 abstract "In order to assess endogenous and colonic production of acetate, we have developed an assay for determining the isotopic enrichment of plasma acetate using gas chromatography/mass spectrometry (GC/MS). Acidified, deproteinized plasma (200 μl) was extracted into ethyl ether, and the ether phase was then injected into a gas chromatograph/mass spectrometer fitted with a 30 m × 0.252 mm i.d. capillary column (temperature program 50–245°C at 10°C min−1). Using electron impact GC/MS and selected ion monitoring, peak areas of ions with m/z 60 and 61 (M + 1) were determined. Triplicate extractions of enriched plasma samples (mol.% excess 1.38–1.5%) resulted in a coefficient of variation of 1.6–5.9%. Unenriched plasma samples were found to have an enrichment close to theoretical natural abundance, and analysis of our (1-13C)acetate tracer (99 at.% excess) revealed an ion at m/z 61 and no ion at m/z 60. To verify accuracy, we conducted an in vivo isotope dilution study. In a 1-month-old piglet, fasted for 24 h, changing the rate of a 4 h infusion (mmol h−1) of (1-13C)acetate from 0.141 to 0.282 doubled the isotope enrichment (2.08 x) of the second plateau. The rate of appearance of acetate was 26.0 μmol kg−1 min −1, which is comparable to that reported in fasting sheep." @default.
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- W2076683313 date "1990-09-01" @default.
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- W2076683313 title "Measurement of stable isotopic enrichment of underivatized acetate by gas chromatography/mass spectrometry: Application toin vivo estimation of acetate production" @default.
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- W2076683313 doi "https://doi.org/10.1002/bms.1200190906" @default.
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