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- W2076690126 abstract "<i>Objectives:</i> To investigate the effect of 5-Aza-CdR on methylation and expression of <i>RASSF1A</i> gene in the human bladder cancer BIU87 cell line. <i>Methods:</i> Cultured BIU87 cells were treated with increasing concentrations (0.1, 0.5, 1.0, 5.0 μmol/l) of 5-Aza-CdR. MTT was used to detect the proliferation of BIU87 cells. The methylation status and expression level of <i>RASSF1A</i> gene in BIU87 cells were analyzed by methylation-specific polymerase chain reaction and RT-PCR before and after treatment with 5-Aza-CdR. Meanwhile, Western blot was used to detect the changes of RASSF1A protein. <i>Results:</i> In this study we found that the growth velocity of the BIU87 cell line was suppressed to various degrees after having been treated with different concentrations of 5-Aza-CdR, and the survival rate decreased with increasing 5- Aza-CdR concentrations. Besides, the methylation status of RASSF1A was obviously reversed and mRNA was re-expressed after treatment with 5-Aza-CdR. Expression of RASSF1A protein increased with increasing 5-Aza-CdR concentrations. <i>Conclusions:</i> Our results suggest that the tumor-suppressive effect of 5-Aza-CdR may result from reactivation of silenced RASSF1A through a demethylation function." @default.
- W2076690126 created "2016-06-24" @default.
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- W2076690126 date "2009-01-01" @default.
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- W2076690126 title "Study of 5-Aza-CdR on Transcription Regulation of <i>RASSF1A</i> Gene in the BIU87 Cell Line" @default.
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- W2076690126 doi "https://doi.org/10.1159/000176036" @default.
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