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- W2076702330 abstract "The choice of medium and supplements can affect meiotic regulation and may have an impact on the regulation of mammalian oocyte growth and embryonic cell function. The aim of the present study was to assess the effects of oxygen concentration and endogenous lanosterol on the in vitro maturation (IVM) media without serum and based on recombinant human chorionic gonadotrophin in prepubertal ewe oocytes. Firstly, the effect of varying oxygen concentrations (5% and 20%) during IVM in TCM-199 supplemented (4 mg/ml bovine serum albumin (BSA), 100 μM cysteamine, 0.3 mM sodium pyruvate, 0.1 UI/ml recombinant follicle-stimulating hormone (r-FSH; Gonal-F® 75 UI, Serono, Italy), 0.1 UI/ml recombinant leuteinizing hormone (r-LH; Lhadi® 75 UI, Serono, Italy) and 1 μg/ml estradiol-17β) on subsequent nuclear maturation of oocytes examined under ultraviolet light following staining with bisbenzimide (Hoechst 33342) was investigated. Secondly, two concentrations of lanosterol (0, 10 and 50 μM) were added to the IVM medium. Nuclear maturation of oocytes was examined as previously. Lipid content in oocytes, an important indicator of cytoplasmic maturity, was also measured using Nile red fluorescent stain. The results showed that low oxygen concentration affected the nuclear maturation. Similarly, a significantly higher rate of meiosis resumption was observed with 10 μM (72.3%) of lanosterol compared with the control (51.8%) or 50 μM of lanosterol (59.4%). A significantly higher content of lipids was also observed with 10 and 50 μM of lanosterol (7.3 ± 0.2 × 10(6) and 7.4 ± 0.2 × 10(6) arbitrary units of fluorescence) compared with the control (6.7 ± 0.2 × 10(6) arbitrary units of fluorescence). The results indicate that 10 μM lanosterol during IVM in medium without serum and based on recombinant human chorionic gonadotrophin has a positive effect on maturation of prepubertal ewe oocytes." @default.
- W2076702330 created "2016-06-24" @default.
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- W2076702330 date "2011-08-15" @default.
- W2076702330 modified "2023-09-23" @default.
- W2076702330 title "Effect of lanosterol on the in vitro maturation in semi-defined culture system of prepubertal ewe oocytes" @default.
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- W2076702330 cites W1968700010 @default.
- W2076702330 cites W1971438031 @default.
- W2076702330 cites W1971821104 @default.
- W2076702330 cites W1973696025 @default.
- W2076702330 cites W1976339009 @default.
- W2076702330 cites W1979282492 @default.
- W2076702330 cites W1981378026 @default.
- W2076702330 cites W1989727348 @default.
- W2076702330 cites W1990255218 @default.
- W2076702330 cites W1990815117 @default.
- W2076702330 cites W1991415652 @default.
- W2076702330 cites W1997800433 @default.
- W2076702330 cites W2001265103 @default.
- W2076702330 cites W2004432714 @default.
- W2076702330 cites W2004908651 @default.
- W2076702330 cites W2005380259 @default.
- W2076702330 cites W2008638863 @default.
- W2076702330 cites W2009044468 @default.
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- W2076702330 cites W2020345573 @default.
- W2076702330 cites W2021265522 @default.
- W2076702330 cites W2033703821 @default.
- W2076702330 cites W2034282657 @default.
- W2076702330 cites W2040156011 @default.
- W2076702330 cites W2043696894 @default.
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- W2076702330 doi "https://doi.org/10.1017/s096719941100044x" @default.
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