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- W2076709076 abstract "Escherichia coli harbouring a recombinant plasmid containing the α-amylase gene (HB101/pHI301) and its derivative which can stably maintain the plasmid (HB101/pHI301A) were cultivated in a mini-jar fermenter. By controlling dissolved oxygen (DO) at a concentration of 2–3 μg/l and supplementing with suitable amounts of nutrients, the organisms could grow to a concentration (up to 40 g dry cell l−1) in a semi-synthetic casamino acid medium. During culture the plasmid was maintained stably in both strains. In a synthetic medium, both micro-organisms grew less well and plasmid-free segregants were observed with HB101/pHI301 during the fed-batch culture. On the other hand, plasmid pHI301A was stably maintained throughout the fed-batch culture of HB101/pHI301A. Growth inhibitors, which may be metabolic products, accumulated in the medium and reduced bacterial growth." @default.
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- W2076709076 date "2007-04-24" @default.
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- W2076709076 title "Effects of nutrients on α-amyiase production and plasmid stability in fed-batch culture of recombinant bacteria" @default.
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