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- W2076724625 abstract "The effects of (1) the presence or absence of androgen in vivo, and (2) treatment with Triton-X-100 in vitro upon some chemical and biochemical properties of rat ventral prostate nuclei, isolated in hypertonic sucrose, are described and compared with results reported for rat liver nuclei. Light- and electron microscopic evidence of nuclear purity, hormone-induced changes in the content of homogenate and nuclear protein, RNA and DNA, and the effect of detergent-washing on the composition of isolated nuclei are presented. Nuclei obtained from castrated rats contained less protein and RNA than those from normal animals, while androgen restored the content of these macromolecules. The association of several marker enzymes with prostate nuclei was examined: nuclei washed with detergent appear to retain cytochrome oxidase, an acid phosphatase and glucose-6-phosphatase activity; with the methods employed lactic dehydrogenase and uricase were not detected. Nuclear RNA, NAD and putative protein synthesis were not adversely affected by detergent. The effect of androgen on this apparent nuclear protein synthesis is being investigated." @default.
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- W2076724625 date "1970-08-01" @default.
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- W2076724625 title "Effects of androgen in vivo on some properties of isolated rat ventral prostate nuclei" @default.
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- W2076724625 doi "https://doi.org/10.1016/0014-4827(70)90460-x" @default.
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