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- W2076750841 abstract "The photoinitiated radical reactions between thiols and alkenes/alkynes (thiol-ene and thiol-yne chemistry) have been applied to a functionalization methodology to produce carbohydrate-presenting surfaces for analyses of biomolecular interactions. Polymer-coated quartz surfaces were functionalized with alkenes or alkynes in a straightforward photochemical procedure utilizing perfluorophenylazide (PFPA) chemistry. The alkene/alkyne surfaces were subsequently allowed to react with carbohydrate thiols in water under UV-irradiation. The reaction can be carried out in a drop of water directly on the surface without photoinitiator, and any disulfide side products were easily washed away after the functionalization process. The resulting carbohydrate-presenting surfaces were evaluated in real-time studies of protein–carbohydrate interactions using a quartz crystal microbalance (QCM) flow-through system with recurring injections of selected lectins, with intermediate regeneration steps using low pH buffer. The resulting methodology proved fast, efficient and scalable to high-throughput analysis formats, and the produced surfaces showed significant protein binding with expected selectivities of the lectins used in the study." @default.
- W2076750841 created "2016-06-24" @default.
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- W2076750841 date "2012-04-01" @default.
- W2076750841 modified "2023-10-17" @default.
- W2076750841 title "Photogenerated lectin sensors produced by thiol-ene/yne photo-click chemistry in aqueous solution" @default.
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- W2076750841 doi "https://doi.org/10.1016/j.bios.2012.01.001" @default.
- W2076750841 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3405975" @default.
- W2076750841 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22341757" @default.
- W2076750841 hasPublicationYear "2012" @default.
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